Cultivation Of Pleurotus Eryngii, Pleurotus Nebrodensis And Hypsizygus Marmoreus Using Asparagus Old Stem

The main aim of this paper is to study the cultivating technology of Pleurotus eryngii, Pleurotus nebrodensis and Hypsizygus marmoreus using asparagus old stem as main substrate, and analyze the polysaccharides content and antioxidant activities of these mushrooms fruit bodies. Shanxi Province currently owns the nation's largest production base of asparagus. Therefore, cultivating mushrooms with asparagus old stem can, on the one hand, enhance the value-added agricultural products and build ecological cycle of agricultural economic structure, in line with the trend of modern agriculture; on the other hand, can fill gaps in cultivation of rare edible mushrooms in Shanxi Province and promote the development of mushroom cultivation technology in this region.The media of five formulas consisted of asparagus old stem, cottonseed hull, bean, soybean cake and gypsum were used to cultivate P. eryngii, P. nebrodensis and H. marmoreus. The polysaccharide content and antioxidant activities of fruit bodies of these mushrooms harvested in the different media were evaluated. Phenol-sulfuric acid method was used to determine the polysaccharides content. Three complementary assays (i.e. reducing power, DPPH radicals scavenging capacity and chelating ability for ferrous ions) were used to screen the antioxidant activities of ethanolic extracts from these mushrooms.Firstly, the yield and biological efficiency of P. eryngii, P. nebrodensis and H. marmoreus were compared under the same cultivation conditions. P. nebrodensis strain B2gave the highest biological efficiency, and H. marmoreus strain Z1gave the lowest biological efficiency. On different media, there were apparent differences in the yield and biological efficiency among P. eryngii, P. nebrodensis and H. marmoreus. The yield and biological efficiency of P. eryngii and H. marmoreus in the medium of Formula4were higher than that in other media. The yield and biological efficiency of P. nebrodensis in the medium of Formula5were higher than that in other media.Secondly, the polysaccharide contents of P. eryngii, P. nebrodensis and H. marmoreus were compared under the same cultivation conditions. Among5P. eryngii strains, strain X2gave the highest polysaccharide content, and strain X3gave the lowest polysaccharide content. Among5P. nebrodensis strains, the polysaccharide content of strain B2was the highest, and that of strain Bl was the lowest. Among5H. marmoreus strains, the polysaccharide content of strain Z2was the highest, and the polysaccharide content of strain Z3was the lowest.The polysaccharides content of P. eryngii strain X2fruit bodies harvested on the medium of Formula2reached its highest value of7.69%. The polysaccharides content of P. nebrodensis strain B2fruit bodies harvested on the medium of Formula4reached its highest value of7.2%. The polysaccharides content of H, marmoreus strains Z1fruit bodies harvested on the medium of Formula2reached its highest value of5.43%. The results illuminated that different media could influence the polysaccharides contents of the fruit bodies of P. eryngii, H. marmoreus and P. nebrodensis, and the polysaccharide contents of P. eryngii, P. nebrodensis and H, marmoreus fruit bodies harvested on asparagus old stem asparagus substrate were significantly higher than that on cottonseed hull substrate.Finally, the antioxidant activities of P. eryngii, P. nebrodensis and H. marmoreus fruit bodies were evaluated by determining the reducing power, DPPH radicals scavenging ability and Fe21-chelation ability. The results showed that there were apparent differences in the antioxidant activities among different strains of these mushrooms. The antioxidant activities of P. eryngii, P. nebrodensis and H. marmoreus fruit bodies harvested on asparagus old stem substrate were significant stronger than that on cottonseed hull substrate.