Photoautotrophic Micropropagation Rooting Technique For Excellent Clones Of Cunninghamia Lanceolata

Cunninghamia Lanceolata belongs to the Taxodiaceae, Cunninghamia, gymnosperms. Itstimber is uniform, light, tough and easy processed. Therefore it is widely used in various aspectsof architecture, furniture, shipbuilding and so on. Because of this it plays an important part inChina's plantation. We did the optical self-raising micro-propagation rooting of CunninghamiaLanceolata research in optical self-raising micro-propagation box which enviromental factorscould be controled. In this research we analyzed rooting index, chlorophyll content, root activity,and chlorophyll fluorescence in the rooting process of Cunninghamia Lanceolata. And we foundthat the best ecological factors such as matrix, rooting accelerator, nutrient solution, lightintensity, CO₂concentration and so on. Ultimately we found a set of efficient, rapid and feasibleCunninghamia Lanceolata rapid propagation technology system which could promote thedevelopment of seedling nursery. The main results are as follows:1We decided the best rooting accelerator by analyzing rooting index of CunninghamiaLanceolata. Rooting effect was best when treated with100mg/L ABT before cutting. Thesurvival rate of Cunninghamia Lanceolata cuttings was84%, rooting rate was91.67%, theaverage root length was3.6cm, and the average number of roots was4.2From analyzing the rooting index of different parts of branches in different matix wefound that the top tips of branches of Cunninghamia Lanceolata in the matix where vermiculite:perlite was2:1had the best rooting. The survival rate of Cunninghamia Lanceolata cuttings was94.25%, rooting rate was95%, the average root length was6.88cm, and the average number ofroots was6.3When treated Cunninghamia Lanceolata with different nutrient solution we found that thebest nutrient solution for rooting rate of Cunninghamia Lanceolata was1/2MS+IBA(0.2mg/L)+NAA(0.1mg/L), the best nutrient solution for root length of Cunninghamia Lanceolata was1/4MS+IBA(1.0mg/L)+NAA(0.5mg/L), and the best nutrient solution for root weight ofCunninghamia Lanceolata was1/2MS+IBA(1.0mg/L)+NAA(0.1mg/L). We determined thebest nutrient solution for rooting from analyzing the results that was1/2MS+IBA(0.2mg/L)+NAA(0.1mg/L)finally.4When treated Cunninghamia Lanceolata with different CO₂concentration and different light intensity we found that the best CO₂concentration and light intensity for CunninghamiaLanceolata rooting was that CO₂concentration was1500ppm, and light intensity was6000lux.In this condition, the rooting rate of Cunninghamia Lanceolata cuttings was97.6%, the averageroot length was6.14cm, the average number of roots was6.8, the average root weights was0.377g, the Chlorophyll content was1.31mg/g, and the root activity was147.55ug.g-1.h-1.5The trend of chlorophyll fluorescence value changes such as Fo values, Fm values wasrising and then declining and rising again. The trend of Fv/Fm value and Fv/Fo value changeswas rising and then declining. The best rising condition was that CO₂concentration was1500ppm, light intensity was6000lux.6we found that cuttings of Cunninghamia Lanceolata under the condition ofphotoautotrophic micropropagation the cuttings rooting rate, root length, root number, rootweight, chlorophyll content and root activity increased by4.95%,30.6%,78.9%,123.9%,26%,36.8%compared with outdoor cuttings of Cunninghamia Lanceolata in the sand bedWhen these cuttings cultivated30days later.