Impacts Of Domestication And Breeding On The Proteomes Of Leaves In Soybean

The leaf proteomic differences among wild, landraces and cultivars populations were analyzed using by2-DE. It was determined that the intensity of27(3.71%)of these protein spots differed significantly among the three populations.1. A total of22proteins were identified from27differentially expressed protein spots by MS/MS. The successful identification rate was81.5%. Of22identified successfully protein spots,15were from legume, the ratio was68.2%.2.13proteins were identified from15(2.06%) differentially expressed protein spots between wild and landraces populations. Of these13protein spots,5were upregulated in domestication, involved in photosynthesis, photorespiration and active oxygen metabolism; and8were downregulated, involved in photorespiration, abiotic stress resistance, vegetative storage, DNA repair.3.13proteins were identified from15(2.06%) differentially expressed protein spots between landrace and cultivar populations. Of these13protein spots,3were upregulated in breeding, involved in photosynthesis, vegetative storage and abiotic stress resistance; and10were downregulated, involved in photosynthesis, photorespiration, vegetative storage, active oxygen metabolism, abiotic stress resistance, vegetative storage and DNA repair.4. It was determined15(2.06%) differentially expressed protein spots between landraces and cultivars. Of these15protein spots,4were upregulated and11were downregulated in cultivars compared to landraces. It was determined20(2.75%) differentially expressed protein spots between wild and cultivars group. Of these20protein spots,5were upregulated and15were downregulated in cultivars compared to landraces. Of27differentially expressed protein spots,2were specific proteins (Nos.7,23) in wild population, and1was specific protein(Nos.3) in landraces population.These results provide a certain foundation for looking for target gene selected artificially of soybean domestication and breeding.