Optimization Of Extraction And Purification Technology For Camellia Saponin

Camellia oleifera was widely grown in the south of China.Camellia saponin was extractedfrom the Camellia seed meal, which was a natural non-ionic surfactant.Because of its excellentsurface activity and biological activity, it was widely used in daily-use chemical and healthcare industries.Camellia oleifera resources were rich in China.But comprehensive utilization ofCamellia oleifera resources was far from enough in China.There was a bright perspect instudying and developing the camellia saponin in tea seed.In this paper, in order to get high-purity camellia saponin, it was extracted from Camelliaseed meal and aqueous phase of tea oil extraction by enzymatic processing.The crude productswere purified by resins chromatography, and optimizated the process.Finally camellia saponinwas further purificated and separated by HSCCC.The main results of research are as follows:1. Ethanol was chose as the extraction solvent of saponin from Camellia seed meal, theorthogonal test was used to confirm the optimal extraction conditions.The results showed thatthe optimum technological conditions were as follows:ethanol concentration50%, soakingtemperature60℃,ratio of liquid to solid1:10and soaking time2h.Under the conditions, thecamellia saponin yield was16.66%, and its purity in crude products was62.37%.2. Using the aqueous phase of tea oil extraction by enzymatic processing as raw material,camellia saponin was extracted by flocculation-precipitation.Based on single factorexperiments, it was found to be better when1%chitosan was used with the amount of16%liquid total volume, the rate camellia saponin loss was22.24%.The orthogonal test was used toconfirm the optimal precipitation-transformation conditions, and the conditions were asfollows:the amount of CaO was3%of the liquid weight, precipitation temperature was20℃,precipitation time was6h; after centrifugation, the ratio of NH4HCO3to CaO was2.5, releasetemperature was40℃,release time was0.5h, under the optimum conditions the content ofsaponin was80.25%.3. Factorial test results indicated that camellia saponin purified by AB-8resin was the bestand the best conditions were studied.The results indicated that AB-8macroporous resin had higher desorption rates and it was applicable for the purification of camellia saponin.Thesample flow velocity was2.6BV/h, the elution solvent was2BV80%ethanol at2BV/h.Thecontent of obtained camellia saponin reached88.5%, and the recovery rate was83.7%.Then wemagnified the experiment according to the optimized conditions.Separated40g crude extractsample once time, could get11.89g camellia saponin with a purity of over96%, and therecovery rate was45.51%.4.Preparative high-speed countercurrent chromatography was used for isolation andpurification of camellia saponin.Several classic solvent systems were screened and adjusted.The preliminary results showed that the solvent system of ethyl acetate-n-butanol–water(1:1:2,V/V)was suitable for the purification of camellia saponin.The lower phase of the systemwas used as the mobile phase at the flow rate of8.0mL/min, the revolution speed was set at560rpm.Finally its content could increase to91.8%.