Study On Extraction And Purification Of Gelsemium Alkaloids

Objective:This experiment compared the extraction method of 95%ethanol,acid water,and water solvent to the extraction rate of total alkaloids from Gynura.Find a simple,efficient,and scientific method to extract from it.Because the macroporous resin method can effectively control the production cost and have a short production cycle,the use of class I or class II organic solvents can be avoided when extracting alkaloids.This is not the case with other methods,so the investigation of the macroporous resin method is necessary.The purification conditions of alkaloids were optimized,which provided a theoretical basis for the industrial purification of hook-and-mouth kisses using the macroporous resin method.Methods:In this study,90%ethanol and 0.5%H₂SO4 were used as solvent,and water was used for extraction of hook-nose alkaloids.The extraction yields of the crude extracts from the different extracts were different.The comparison provides a reference for future industrial production of hook-and-hook.In order to further improve the utilization of hook and kiss.Further purification of the crude extract of the hooked snout is a critical step,so this test was performed by preliminarily testing the hookah alkaloid eluate with the following four factors:sample pH?A?,30%ethanol rinse?B?,100%ethanol eluate?C?and extract stage?D?,conduct four-factor three-level orthogonal test design L9^{?3^4?},explore the macroporous resin method to optimize the purification of hook-nose alkaloids condition.At the same time,this experiment selected Xiao Sha?2016?master's degree thesis“The determination of the crude extracts of hook-and-mosquito extracts and the in vitro metabolism studies of major alkaloids”was performed to determine the content of crotin and hookin in the crude extract of The method comprises the following steps:measuring the content of Crosminin and Crocin A in a sample of H.crocea alkaloids purified by a macroporous resin method.Results:From this comparative test,the alkaloid content of alcohol extract>acid aqueous extract>water extract after the crude extraction of hook and kiss was found,and the percentages of alkaloids in the three solvent extraction methods were 2.15,6.44,and 0.57,respectively.From the results of the range analysis and variance analysis of the orthogonal test,it can be concluded that the purification of the hook-nose alkaloids is affected by the ethanol concentration in the washing liquid,the ethanol concentration in the eluent,and the pH value.According to the linear regression equation of the absorbance value and the standard amount of konjac prime:y=0.0055x+0.2236?R2=0.98565?,the purity of the kohlrabi alkaloid macroporous resin was 92%.Simultaneously,high-performance liquid chromatographic method was used to detect the content of crocin methyl in the purified sample of macroporous resin was 24.65%,the content of ghrelin was 16.39%,and the content of ghreloxon A>ghrelin.Discussion:The comparison test showed that after the crude extraction of hook kiss,among them,the alcohol extract with the content of alkaloid>acid water extract>water extract.The yield of crude extract of hook kiss from different solvents was different.From the poor of the orthogonal experiment and variance analysis results can be concluded that the macroporous resin method for the optimal combination of the hook kiss alkaloids purified for A1,B2,C3,and D2,namely sample adjustment pH8,rinses for 30%ethanol,eluent is 100%ethanol and the second phase extraction solution is the optimal combination.The optimal combination of a sample of chondrine alkaloids purification separation purity can reach 92%.Analysis to detect the macroporous resin experiment samples obtained the optimal purification conditions of alkaloids hook kiss a content of 24.65%,hook kiss son of content is 16.39%,among them the test a hook hook kiss kiss alkaloid in content is higher than the hook kiss the child.This result is inconsistent with the opinion of yu changxi et al.?2012?that the content of leptospirotein is the highest in domestic leptospirotein alkaloids.