The Distribution Of SA Receptors In Chicken Oviduct And Pathological Observation With H9N2Avain Influenza Virus

H9N2subtype avian infiuenza(AI) belongs to low pathogenic avian influenza, but itsinfectivity is strong and the genetic variation is fast. In recent years, H9N2AI often occurs inthe layers and concentrates in the time of beginning laying egg or at the speak laying. Theinfected layers produce down, the shells color becomes shallow and deformity eggs, sand, oreggs with soft shells increasing, causing huge economic losses. Dissected the infected layerscan find follicular is congestion, hemorrhage, necrosis, deformation, broken and oviduct isedema and white plastic frozen samples or cheese kind material in oviduct. However, themechanism of H9N2AIV infection layers causeing chicken oviduct abnormal functionresearch is not clear. In this study, the distribution of sialic acids α-2,3Gal and α-2,6Galreceptors in oviduct of layers was investigated by using lectin histochemical staining; H9N2AIV was inoculated to observe histopathological changes of oviduct; epithelial primary cellsof hen magnum were cultured with collagenase I digestion, pathological changes wereobserved after inoculating H9N2AIV. Our purpose is to study the mechanism H9N2AIVpathogenicity for oviduct of layers.(1)Five unimmuned with H9N2AIV vaccine layers were killed by neck bloodletting andthe oviduct were taken. Infundibulum, magnum, isthmus, shell gland and vagina were cut offand maked paraffin section. Then, the distribution of sialic acids α-2,3Gal and α-2,6Galreceptors in oviduct of layers was investigated by using lectin histochemical staining.(2)Twelve unimmuned with H9N2AIV vaccine layers were infected H9N2AIV withmethod of intranasal instillation and eye dropping. The symptoms of infection chickenobservation every day. Every tow layers were killed after infection3d,7d,11d,15d,19d andmaked paraffin section. Histopathological changes of oviduct were observed after HE dyeing.(3) Eighteen weeks unimmuned with H9N2AIV vaccine hens were killed by neckbloodletting. The magnum of oviduct were taken sterilly. Callagenase I was poured intooviduct and digested epithelial primary cells.Then, the primary cells were cultured. H9N2AIV was inoculated when the cells had grown basicly single-layer and pathological changeswere observed after inoculating H9N2AIV72h.