Study On The Semiochemicais Of The Japanese Pine Sawyer, Monochamus Alternatus Hope And Development Of High Effective Lure And Traps

The beetle Monochamus alternatus Hope (Coleoptera: Cerambycidae) is an principal vector ofpinewood nematode, the causal pathogen of pine wilt disease, that has resulted in devastating losses ofpines in much of Asia. But there still wasn't any kind of effective prevention and control measures forthis notorious pest in the international. The chemical communication between sawyer and the host-plantand the relationship between male and female adult weren't clear even now. This paper focus on thechemistry communication of the pine sawyer in host-plant location and mate location in the naturalspread; and the visual cues of adult parasitical in host location and mate location; based on theseresearch we will development of more effective attractant-baited and improved the trap. The resultswere as follows:1. The chemistry communication between the pine sawyer and host-plantAll the field results showed that,(+)-α-pinene was the main compound to M. alternatus, and(+)-α-pinene was most principal compound of the host-plant pinus massoniana volatile. It wasconfirmed that (+)-α-pinene was a key compound in the chemistry communication between the pinesawyer and host-plant. Ethanol and acetaldehyde were causing significant increase in attraction; theyalso appeared to be an important synergistic compound in the process of selection host-plant. The lureshow the strongest attraction to the M. alternatus when the ratio of the mixture of ethanol oracetaldehyde and (+)-α-pinene is1:3. It was testify that, the olfaction organ of M. alternatus was verysensitive for the ratio of host plant volatile compound. And there was a great difference in percentageof volatile compound of the plant in different healthy stage. And the lure of host volatile compoundtempted to more male than female; So we speculate that male M. alternatus are more responsive tothe plant volatiles than females and have more capacity to the mate location. The behavioral traits ofM. alternatus are consistent with the hypothesis that mate location of M. alternatus involves threebehavioral stages.2. The chemistry communication between the male and female M. alternatusIn the field experiment, there was no significant difference with the lure only with malepheromone or plant volatiles. But the lure with both male pheromone and plant volatiles significantincreased the attractants effect; tempted to5times M. alternatus than plant volatiles lure or male pheromone lure. The male pheromone lure and the lure with both male pheromone and plant volatileswere significant tempted to more female than male. But the attractants effect to M. alternatus offemale contact pheromone was very weak, and even lower the plant volatiles lure. It is indicate thatthe female contact pheromone is not a gathered the information of meat. This was also sustained thetheory that mate location of M. alternatus involves three behavioral stages.3. Effect of visual cues on the host location and mate location of the M. alternatusIn the field trapping of M. alternatus, of the four treatments tested, the effect of black and browntraps was significantly stronger than the control, gray traps. But the effect of green traps wassignificantly weaker than the control. In mating behavior assays, after the M. alternatus' visual organcompound eyes were treated, the percentage of mating success significantly decreased to50.67%compared to the control with average93.33%. And proportion of mating success after treatingolfaction was with average72.33%, and it was also significantly lower than the control. It was withaverage26.67%after both vision and olfaction were treated. It indicates that visual cues are ratherimportant in the mating behavior of M. alternatus in short distance. This was also supply the theorythat mate location of M. alternatus involves three behavioral stages.All in all, this study were great added the theory of the chemical communication in the parasiticalspread in natural; Expounded the mechanism of the chemical communication between M. alternatusand the host, and the chemical communication between male and female adult, and the visual cues onthe host location and mate location. Then, we development of the lure and improved the trap based onthese result. The next promotion, we need use the achievement to the whole province and even thenational for prevention and control the M. alternatus and pinewood nematode.