Association Studies Between Polymorphisms In Proly Isomerase Pin1 And The Risk For Laryngeal Squamous Cell Carcinoma

ObjectiveTo ascertain the relationship between polymorphisms and laryngeal squamous cell carcinoma(LSCC)occurrence and development, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) was used to detected the polymorphisms of Pinl (peptidyl-prolyl cis/trans isomerase 1)in a hospital-based case-control study of patients with LSCC and cancer-free control subjects. Three selected Pinl polymorphisms (rs2233678,rs2233679and rs2287838) were genotyped. The genotype frequencies and allele type frequencies were compared, and the relationships between their genotype frequencies and clinical characteristics (cervical lymph node metastasis and clinical status) were seeked respectively.MethodsThe polymorphisms (rs2233678 and rs2233679 in the promoter regions; rs2287838 in the intron regions) of Pinl in peripheral blood of a hospital-based case-control study of patients with LSCC and cancer-free control subjects were detected by the PCR-RFLP method. Differences in the clinical characeristics and frequencies of the Pinl genotypes between the cases and controls were evaluated by using the (?)2 test. The associations between Pinl variants and LSCC risk were estimated by computing the odds ratios (ORs) and 95%confidence intervals (CIs). P<0.05 is regarded as the difference has statistical significance.Results1. The observed genotype frequencies for these three SNPs were all in Hardy-Weinberg equilibrium in the control subjects. Illustrate that the genotype frequencies of these three SNPs in balance, with groups representative.2. The genotype frequencies and allele type frequencies of rs2233678 were significantly different in LSCC patients (P<0.05), which was not related to cervical lymph node metastasis and clinical status (P>0.05). Compared with the rs2233678 GC+CC genotype, the rs2233678 GG genotype was 3.821 times more associated with a significantly increased LSCC risk (P=0.001, OR=3.821, 95%CI=1.632-8.948); and the rs2233678 G allele type also with a significantly increased LSCC risk than C allele type (P=0.001, OR=3.858,95%CI=1.716-8.673).3. The genotype frequencies and allele type frequencies of rs2233679 have no statistical significance (P>0.05).4. The genotype frequencies and allele type frequencies of rs2287838 were significantly different in LSCC patients (P<0.05), which was not related to cervical lymph node metastasis and clinical status (P>0.05). Compared with the rs2287838 TT genotype, the rs2287838 CT genotype was associated with a significantly decreased LSCC risk (P=0.000,OR=0.141,95%CI=0.060-0.330); and the rs2287838 CC genotype with a non-significantly decreased LSCC risk (P=0.059, OR=1.470,95%CI=0.985-2.193)Conclusion1. The rs2233678 variant genotypes were associated with LSCC risk, which was not related to cervical lymph node metastasis and clinical status. The rs2233678 G mutant allele was associated with a significantly increased LSCC risk.2. No associations were observed between the variants rs2233679 and LSCC risk.3. The rs2287838 variant genotypes were associated with LSCC risk, which was not related to cervical lymph node metastasis and clinical status. The rs2287838 TT genotype was associated with a significantly increased LSCC risk.