| Background and objectiveNowdays, malignant tumor has become a serious threat to human's health, it has impacts on the quality of the life of the patients. Especially for the malignant tumor, which is difficult for early diagnosis, invasion and metastasis quickly and has a low 5-year's survival rate aggravatedthe humans burden. Gastric carcinoma is one of the most common malignancies, which is threaten the human's health, every year there are about 7 million people died of gastric carcinoma. In the world's wide gastric carcinoma's death rate only below lung cancer, colorectal and breast cancer. Gastric carcinoma's genetic mechanism is very complicated, at present the gene detects is not application in gastric cancer clinical practice. Along with the progress of gastric molecular pathology, people know to cancer of the stomach increased significantly. Conduct genetic test, new biomarkers based on the genetic research has been widely used in scientific research fields.Single nucleotide polymorphisms are the third generation of genetic markers, are the most important genetic change in humans. SNPs have characters of large quantity, stabilization and feasibility for detecting. At present, People have paid an importance to SNPs and the relation between SNPs and genetic susceptibility to disease, sensibility to medicine, prognosis have been researched. The relationship between SNPs of certain genes and certain carcinomas has been confirmed.CDH17 namely cadherin 17, also known as LI-cadherin or liver-intestine cadherin located in 8q22.1, its a special member of cadherin superfamily. It is a gastric cancer related gene, which has its own characteristics in mediated cell adhesion. This experimental analysis CDH17 gene explicit son 6A58G site and explicit son 17A49C site genotype distribution frequency and allele frequency type distribution, aims to study the correlation between the two sites and gastric carcinoma crowd's in China henan, so as to provide theory basis for the development of gastric carcinoma.Materials and methods1. The peripheral venous blood samples were collected from 68 unrelated patients with gastricl carcinoma in Henan province, China. The time of collecting samples were from December 2009 to April 2010. All patients with gastric carcinoma had treatments in the first affiliated hospital of Zhengzhou University.1mL Blood samples were collected from patients before surgery.2. DNA extraction kits of spin column type was used to extract DNA from sixty-eight peripheral blood samples from patients with gastric carcinoma.3. The method of the polymerase chain reaction-conformation polymorphism single-strand (PCR-SSCP) was used to detect genotype, combined with DNA sequencing was used to check the correctness of genotype4. The methods of statistical analysis:SPSS 17.0 statistical software package was used. About unordered categorical datas, analyse them byχ2 test, Odds ratio(OR) and 95% confidence interval(95%CI) were used to express risk degree(OR and 95%CI were computed by Logistic regression model). About ordered categorical datas, analyse them by rank sum test, the mean ranks were used to express risk degree. Using two-sides probability test when there were two kinds of side tests. Significant level was 0.05. Method of Bonferroni was used to mutiple comparision. Results1. The genotype frequencies of CDH17 extron 6 A58G site:A/G type has 52 cases (76.47%), G/G type 11 patients (16.18%), A/A type five cases (7.35%). The allele frequencies of CDH17 exon 6 A and G were 45.59%,54.41% respectively.2. The genotype frequencies and allele frequencies of CDH17 extron 6 A58G site has no significant correlation with the gender, age, size, invasion depth, macroscopic appearance, histologic type and differentiation of gastric carcinoma (P> 0.05). The genotype frequencies of CDH17 extron 6 A58G site has correlation with lymph node metastasis and TNM grade of gastric carcinoma (P<0.05)3. The genotype frequencies of CDH17exon17 A49C:genotype A/C26 cases (38.24%), genotype A/A 17 cases (25.00%), genetic type C/C25 cases (36.76%). The allele frequencies of CDH17 exon17 A and C were 44.12%,55.88% respectively.4. The genotype frequencies and allele frequencies of CDH17exon17 A49C site has no significant correlation with the gender, age, size, invasion depth, macroscopic appearance, histologic type, differentiation, lymph node metastasis and TNM grade in patients with gastric carcinoma.(P>0.05),Conclusions1. The genotype frequencies and of CDH17 extron 6 A58G site has no significant correlation with the gender, age, size, invasion depth, macroscopic appearance, histologic type and differentiation of gastric carcinoma. The allele frequencies of CDH17 extron 6 A58G site has no significant correlation with the gender, age, size, invasion depth, macroscopic appearance, histologic type, differentiation, lymph node metastasis and TNM grade of gastric carcinoma, this site of SNPs may be lead to functional changes of gastric cancer metastasis-related protein.2. The genotype frequencies and allele frequencies of CDH17exonl7 A49C site has no significant correlation with the gender, age, size, invasion depth, macroscopic appearance, histologic type, differentiation, lymph node metastasis and TNM grade in patients with gastric carcinoma.3. The genotype frequencies and of CDH17 extron 6 A58G site has correlation with lymph node metastasis and TNM grade in patients with gastric carcinoma... |
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