The Expression Of ER,VEGF And ENS In Endometrial Polyps And Its Clinical Significance

Background and PurposeAs one of common gynecologic diseases, Endometrial polyps is the main pathogeny of abnormal uterine bleeding. In recent years, with the improvement of living level and the development of modem diagnostic techniques, its morbidity is increasing year by year. So far the exact pathogeny and pathogenesis about Endometrial polyps is not certain completely. Related research show that long-term Estrogen continuous stimulation is related to Endometrial abnormal proliferation and closely connected with Endometrial tissue abnormalities angiogenesis. Therefore, it is helpful to understanding the mechanism of EP occurrence and development to explore related factors with endometrial proliferation angiogenesis.Estrogen plays biological action by combining with Estrogen-receptor, on the one hand, it mediates the expression of VEGF to realize propelling the cyclical reconstruction of endometrial blood vessel; on the other hand,it promotes endometrial Hyperplasia and repair. Vascular endothelial growth factor is the main growth factor to promote angiogenesis. It can act on Vascular endothelial cells through its receptor specificit. VEGF has two functions:not only promote the Vascular endothelial cells to divide and proliferate but also increase vascular permeability. VEGF not only engages the process of endometrial Angiogenesis in menstrual cycle,but also is closely related to pathological process of excessive angiogenesis.Endostatin is the strongest endogenous angiogenesis inhibiting factor at present. It can specifically inhibit the migration and proliferation of endothelial cells caused by VEGF and bFGF,Also it can induce the apoptosis of endothelial cell,but it can not inhibit the migration and proliferation of non-endothelial cell.At present, there is no report of joint detection of ER, VEGF and ENS in EP patients. This research discusses impact of ER and Angiogenesis adjustment factors in EP occurrence and provide theoretical basis for prevention and treatment of EP by determining expression of ER,VEGF and ENS in Endometrial polyps and normal endometrial tissue.Material and Methods1.GroupingEndometrial polyps Group:Take typical polyp tissue samples in the 50 patients dignosed as Endometrial polyps in department of gynaecology and obstetrics of the second affiliated hospital of Zhengzhou University from Octorber 2009 to July 2010.Peripolyps endometrium Group:Take endometrium around polyps within 0.5-1.0 centimeters which are verifyied to be endometrial hyperplasia by pathological in the same 50 patients dignosed as Endometrial polyps.Normal Endometrium Group:Take normal endometrium in the 50 patients of oviductal problem in department of gynaecology and obstetrics of the second affiliated hospital of Zhengzhou University from Octorber 2009 to July 2010. Take normal intima in proliferative stage proved by pathology.2.MethodsWith RT-PCR and Immunohistochemistry SP method, detect the mRNA and protein expression of ER,VEGF and ENS in three groups quantitatively and qualitatively.3.Statistical analysis Using SPSS package for windows 17.0 statistical analysis to test all data.The expression of ER,VEGF and ENS in three gourps is compared by Oneway ANOVA Different binary comparison between the groups uses the LSD-t test.The correlation between groups is tested by Pearson correlation analysis, the level for significance tests is 0.05.Results1. Expression of ER(1) The mRNA and protein expression of ER in Endometrial polyps Group is higher than that in Peripolyps endometrium Group.The difference is significant statistically (P< 0.05). The mRNA and protein expression of ER in Endometrial polyps Group is higher than that in Normal Endometrium Group.The difference is significant statistically (P< 0.05).(2) The expression of ER in Peripolyps endometrium Group is higher than that in Normal Endometrium Group.The difference is significant statistically (P<0.05).2. Expression of VEGF(1) The expression of VEGF in Endometrial polyps Group is higher than that in Peripolyps endometrium Group.The difference is significant statistically (P<0.05).The expression of VEGF in Endometrial polyps Group is higher than that in Normal Endometrium Group.The difference is significant statistically (P<0.05).(2) The expression of VEGF in Peripolyps endometrium Group is higher than that in Normal Endometrium Group.The difference is significant statistically (P<0.05).3. Expression of ENS(1) The expression of ENS in Endometrial polyps Group is higher than that in Peripolyps endometrium Group.The difference is significant statistically (P<0.05).The expression of ENS in Endometrial polyps Group is higher than that in Normal Endometrium Group.The difference is significant statistically (P<0.05).(2) The expression of ENS in Peripolyps endometrium Group is higher than that in Normal Endometrium Group. The difference is significant statistically (P<0.05).4. Relationship between ER and VEGFThere is a positive correlation between the expression of ER and the expression of 5. Relationship between VEGF and ENSThere is a positive correlation between the expression of VEGF and the expression of ENS in Endometrial polyps (P<0.05, r=0.536)。Conclusions1. ER is higher expression in Endometrial polyps,which related to the formation of Endometrial polyps.2. The expression of VEGF is higher and positively related to that of ER,both of them acting on each other and promoting the development of Endometrial polyps.3. Antiangiogenic by VEGF may open up new ways in the treatment of endometrial polyps.4. High-level expression of ENS in Endometrial polyps tissue is positively related to VEGF expression, which implies it is connected with body compensatory regulation.