The Expression And Significance Of TGF-β₁ And CyclinD1 In Parathyroid Tumors

BackgroundParathyroid is the important endocrine gland in human, the secretion of parathyroid hormone (PTH) is the the most important hormones in human body which adjust blood calcium level. Parathyroid tumors most have the typical symptoms of primary hyperparathyroidism, the main clinical type is bone and bone-kidney mixed type, some of them show other symptoms, such as nausea, gastrointestinal symptoms, inappetence, emaciation, etc. It is often a lack of typical clinical manifestations in early stages.The main principle is detecting and treating in time.With the research of all kinds of tumor molecular pathogenesis,the research of parathyroid tumors also made some progress. Being a potent negative regulator of cell proliferation, transforming growth factor-β1(TGF-β1) plays an important role in inhibiting normal epithelial cell growth,and it has a complicated relationship with tumors. CyclinD1 is a key protein in G1 phage of the cell cycle. Its over expression can shorten the proeess from G1 to S phage, thus accelerate cell proliferation. In order to investigate the possible mechanism of parathyroid tumors and the clinicoPathologic significance of TGF-β1 and CyclinDl,the exression in parathyroid tumors, an Immunohistochemical SP method was used to examine the expression of the two kinds of proteins.ObjectiveTo investigate both the function of TGF-β1,CyclinD1 and the relationship between the two protein in parathyroid tumors and normal parathyroid tissues. It was helpful in learning the relationship between TGF-β1 signaling pathway and emergence development through study the TGF-β1 and CyclinD1 in parathyroid tumors. It also would provide the basis for diagnosis, judging to stage ofparathyroid tumors' invasion and predicting the prognosis, finding new methods of therapy.MethodsTissues specimens were collected from the First Affiliated Hospital of Zhengzhou University from 1997 to 2009,10 cases of normal parathyroid tissues,43 cases of parathyroid adnoma tissues and 6 cases of parathyroid carcinoma tissues. SP Immunohistochemical technique was used to determine the expression of TGF-β1 and CyclinD1 in normal parathyroid tissues,parathyroid adenoma tissues and parathyroid carcinoma tissues. The data was analyzed by software SPSS 16.0.ResultsThere are 4 positive TGF-β1 expression (40%) and 1 overexpression of CyclinD1 (10%) in 10 normal parathyroid tissuess. There are 21 positive TGF-β1 expression (48.8%) and 24 overexpression of CyclinD1 (55.8%) in 43 parathyroid adenoma tissuess. There are 4 positive TGF-β1 expression (66.7%) and 5 overexpression of CyclinDl (83.3%) in 6 parathyroid carcinoma tissuess.The expression of TGF-β1 was not significantly different among the three groups.The expression of CyclinD1 protein was significantly different between normal parathyroid tissuess and parathyroid adnoma tissuess (P<0.05),and it was significantly different between normal parathyroid tissuess and parathyroid carcinoma tissuess(P<0.05),but it was not significantly different between parathyroid adnoma tissuess and parathyroid carcinoma tissuess (P>0.05). The expression of TGF-β1 was significantly related to the overexpression of CyclinD1 in the parathyroid adnoma tissuess, but it was not significantly related to the overexpression of CyclinD1 in the parathyroid carcinoma tissues. ConclusionsDerangements of the TGF-β1 pathyway due to CyclinD1 would be also implicated in the development of parathyroid tumors, the overexpression of CyclinD1 plays an important role in parathyroid tumors.