Expression Of Survivin,CyclinD1 And PTEN Protein In Gastrointestinal Stromal Tumors And Its Significance

Objective: The expression of Survivin, Cyclin D1 and PTEN proteins in gastro-intestinal stromal tumors (GISTs) was been observed . Meanwhile the relationship between 3 proteins and some clinical parameters were been analyzed to identify whether 3 proteins take in GISTS's development. Methods: 60 cases of GISTs were divided into two groups: high(29cases) and low risk (31 cases) according to the volume of the tumor and the mitotic count. 40 cases normal tissues respectively from oesophagus, stomach, colon and small intestine were taken as control group. All specimens were paraffin-embeded and HE stained. The GISTs were diagnosed by CD117, CD34, actin, vimentin and S-100 immunohistochemistry stain. The expression of Survivin, Cyclin D1 and PTEN were detected by immunohistochemistry method (S-P method). The results and clinical data were analyzed with chi square test, Bivariate Correlation and mutinomial logistic regression with SPSS10.0 software. Results: (1) The expressions of Survivin was correlated with Cyclin D1 in GISTs(r_L=0.53,P_L=0.003; r_H=0.55, P_H=0.04). (2) Both Survivin and Cyclin D1 were not correlated with PTEN(P>0.05). (3) The expressions of Survivin and Cyclin D1 were significant higher in GISTs than in normal group (x~2=33.95, P< 0.01, x~2=33.80, P<0.01) , that also be in high risk group than in low risk group (Z=31.24, P <0.01, Z=20.03, P<0.01). (4) The expression of PTEN was significant higher in high risk group than in normal risk group (μ=7.43, P<0.001) , but no difference was observed between low risk group and normal group ( μ=1.86, P>0.05) . (5) Clinically, the bigger of tumor, the more of mitotic and the lower of the tumor located, the higher of Survivin and Cyclin D1 and the lower of PTEN expression (P<0.01) . (6)No different expression of 3 proteins existed between male and female patients. Conclusion: (1) The increasing expression of Survivin and Cyclin D and decreasing expression of PTEN imply that these molecular events might play an important role in the pathologic morphology of GISTs . (2) Survivin and Cyclin D1 might synergically effect on tumorigenesis of GISTs for their Correlative over expression in both low and high risk groups of GISTs. (3) PTEN might be a late molecular event in GISTs for it's decrease occuring in high instead of low risk group. It might be changed by a signal translating way which is independent from that of Survivin and CyclinD1, because no correlative expression of PTEN with both Survivin and CyclinD1 was been observed. (4) Survivin and CyclinD1 may be taken as the parameters to judge the invasive risk of GISTs clinically.