| Background and ObjectiveCABG (coronary artery bypass grafting, CABG) is one of the important treatments for coronary heart disease. Especially for patients with multi-vessel or intracardiac malformation. The great saphenous vein is widely used in clinical practice as the primary material of CABG, because the great saphenous vein is superficial and easily obtained, and has enough length to connect the aorta and the far coronary artery. The autogenous vein will be restenosis after vein grafts because of endometrial injury by operative, hemodynamic changes and a series of inflammatory factors release. That make the saphenous vein is restricted in the clinical application. In recent years, a series of methods was used for preventing vein restenosis in clinical, such as drug treatment, gene therapy, extravascular stent, the improve expansion fluid and endothelial progenitor cells, but the effect was so poor. It was reported that: Ginsenoside Rbl has some positive effect on preventing vein restenosis. After established the animal models of vein grafting, we used a certain dose of Ginsenoside Rbl by intraperitoneal injection for the animal models. By observed the changes of vein past-grafting, platelet-derived growth factor (PDGF) and nitric oxide synthase (eNOS) in endometrial cells, we found that the ginsenoside Rbl has some positive effect on the vein graft restenosis inhibition. This was a basis theoretical for future clinical application.Materials and Methods45 New Zealand rabbits were randomly divided into three groups experimental group, model group, control group,15 rabbits in each group, "no-touch" technology was used for getting the external jugular vein. Establish the animal model with using a continuous way anastomosis external jugular vein to ipsilateral carotid artery by end-to-end fashion. After 4 weeks, grafting vein was obtained as sample, the vein intimal thickness, the expression of PDGF and eNOS gene was examined by RT-PCR.Results1. The degree of grafted vein stenosisThe picture under-microscope showed that:After 4 weeks, the vascular intimal thickness of experimental group, model group and control group was(44.45±3.10)μm, (51.32±2.61)u.m,(41.52±2.62)μm, the comparison among groups P＜0.05, experimental group, model group and control group were significantly different among groups.2. The results observed by immunohistochemical stainingeNOS, PDGF positive cells can be seen in each group grafted vein. After 4 weeks, the experimental group, model group, the control group expression of positive PDGF index were (35.38±2.72),(40.12±3.77),(27.17±2.88), compared among the groups P<0.05. the expression of experimental group, model group and control group were significantly different among groups, the experimental group, model group, the control group expression of positive eNOS index were (56.92±2.56),(50.80±3.67),(43.95±2.60), compared among the groups P<0.05. the expression of experimental group, model group and control group were significantly different among groups. 3. The results observed by RT-PCR of grafted veinThe expression of eNOS, PDGF gene can be seen in each group grafted vein, the expression of PDGF relative coefficients in experimental group, model group, control group were (1.97±0.09),(2.19±0.06),(1.62±0.09), the expression of PDGF relative coefficient in the model group is higher than the experimental group and control group, compared among groups P<0.05, the expression of experimental group, model group and control group were significantly different among groups. The expression of eNOS relative coefficients in experimental group, model group, control group were (2.18±0.07),(1.92±0.06),(1.63±0.08), the expression of eNOS relative coefficient in the experimental group is higher than in the model group and control group, compared among groups P<0.05, the expression of experimental group, model group and control group were significantly different between groups.4. The results of the correlationThe results calculated by SPSS are that:eNOS is negatively correlated with intimal thickness and that is correlation coefficient is-0.469; PDGF is positive correlated with intimal thickness and that is correlation coefficient is 0.329.Conclusion1. Grafted vein stenosis is negative correlationg with eNOS gene expression and positive correlation with PDGF gene expression.2. Ginsenoside Rbl can promote NOS gene expression and inhibit PDGF gene expression in grafted vein, which related to its effects. |
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