| Research background and objective:The human bone marrow consists of a heterogenous group of cells, including hematopoietic stem cells,endothelialcells, fibroblasts, adipocytes, and osteogenic cells.Additionally, in recent years, another group of cells has been found which are able to proliferate and self-renew,as well as differentiate into cells of the mesenchym such as osteoblasts,chondrocytes, and adipocytes. These cells werecalled mesenchymal stem cells (MSCs), The interest in MSCs has risen since there is hope to usethese cells in regenerative medicine,for example, in acute myocardial infarction and cardiovascular disease, diabetes,stroke, kidney disease, or immunomodulatory disease suchas graft-versus-host disease (GvHD).Methods Rat bone marrow cells were separated and expanded by adherence cultureo The two groups'MSCs of primary were respectively cultured by adhering in L-DMEM containing 15% and 10% FBS. After passaged,all were cultured by adhering in L-DMEM containing 10% FBS. The morphologic features,the growth curve and the surface markers CD44,CD45 and CD90 of MSCs were observed. Results MSCs of primary culture in 15% FBS had more rapid proliferation,earlier colony confluence and shorter time for passage primary (about 8 days) and passage 1 (about 5 days)than in 10% FBS.The growth curves of passage 3 MSCs showed a similar total cell number between the two groups(P>0.05).Two groups'MSCs afterpassaged all expressed CD44 and CD90,lacked expressed CD45.Conclusion The primary MSCs cultured in L-DMEM containing 15% FBS can provide MSCs in shorter period compared with the 10% FBS by adherence culture. |
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