Evaluation Of Antidiabetic Effect Of Ginsenoside Rb1 Based On FFA Overflow In Adipocyte

"Free fatty acid overflow hypothesis in adipocyte"—an important pathogenesis of type 2 diabetes, is used as an important target of drug treatment of diabetes mellitus development. Basing on previous research, taking free fatty acid overflow as the contact point, adipocyte cell culture in Vivo and obese model mice experiments in vitro were used to study the mechanism of ginsenoside Rb1, which is the active ingredient of anti-diabetic drugs ginseng. Using laser scanning confocal microscope, Western blot and other molecular biology methods, the subject studied ginsenoside Rb1 which influence FFAs spillover from fat cells at basis or the insulin resistance state, clarifies antidiabetic mechanism of Rbl.Objective:Studying the mechanism of ginsenoside Rbl to free fatty acid overflow from fat cells by observing the effect of ginsenoside Rb1 on 3T3-L1 adipocyte in vitro and in obese mice induced by Fat diet in vivo.Methods:(1)Observing Ginsenoside Rbl that influences Free fatty acid overflow of 3T3-L1 adipocyte in basis and insulin resistance state. (2) Studying the expression of Perilipin in insulin resistance state 3T3-L1 adipocyte by Western blot. (3)Researching the effect of ginsenoside Rb1 on Perilipin protein translocation in normal state in adipocyte using laser scanning confocal microscope. (4)Researching the effect of ginsenoside Rb1 on the epididymal fat content/body weight,NEFA, TC, TG, HDL-C, LDL-C,TNF-a, IL-6 in obese mice.Results:(1) The study show that Ginsenoside Rb1 neither have significant inhibition on basal 3T3-L1 adipocyte lipolysis, nor remarkable increment of lipolysis caused by TNF-a in 3T3-L1 adipocyte in insulin resistance state; (2) TNF-a induced insulin resistance state in 3T3-L1 adipocyte, brought about Perilipin expression decreased significantly. After the intervention of Ginsenoside Rbl for 24 hours, it reversed down-regulated expression of Perilipin caused by TNF-a in 3T3-L1 adipocyte; (3) Rb1 significantly increased the Perilipin protein translocation from cytoplasm to lipid droplets surface in normal state in 3T3-L1 adipocyte. (4) Ginsenoside Rb1 significantly reduced the epididymal fat content/body weight in obese mice; ginsenoside Rbl had no significant effect on NEFA, TC, TG, HDL-C, LDL-C levels; ginsenoside Rb1 can improve insulin resistance in obese mice and decrease TNF-a, IL-6 level of obese mice; ginsenoside Rbl upregulated the phosphorylation level of HSL,had no significant effect on the expression of HSL in epididymal fat in obese mice.Conclusion:(1) Ginsenoside Rbl may inhibit adipocyte lipolysis in normal state in 3T3-L1 adipocyte to a certain extent; (2) Ginsenoside Rbl may inhibit the lipolysis level increasement caused by TNF-a in 3T3-L1 adipocyte; (3) Ginsenoside Rbl may inhibit lipolysis under basal condition by promoting fat cells translocation of Perilipin from cytoplasm to Lipid droplet surface; may be passed against TNF-a expression caused by the reduction in Perilipin inhibited lipolysis insulin resistance; (4) Ginsenoside Rbl may reduce NEFA levels, fat in obese mice, improving lipid metabolism, improved insulin resistance in obese mice, inhibiting TNF-a, IL-6 and other inflammatory cytokines.ginsenoside Rb1 inhibited lipolysis by upregulating the phosphorylation level of HSL...