The Impact Of Apoptosis Gene Expression On Human Ovarian Cancer Cell Line SKOV3 Treated With The Drug Serum Of Li Chong Sheng Sui Yin

Objective:Using gene chip observed the role of management generations Chinese herbal compound drug serum Li Chong Sheng Sui Yin gene expression profile of ovarian cancer cell line SKOV3,Screen the different expressed genes,investigate the mechanism from aspects of apoptosis of Chinese herbal formula Li Chong Sheng Sui Yin on human ovarian cancer cell line SKOV3.Methods:Wistar rats were divited into two group at random and treated with drugs,prepared the drug serum.Extracted the mRNA from the control group and LCSSB group tumor cell, prepared DNA probe and marked them with Two fluorescent dyes,Cy3 and Cy5, hybridized with the cDNA microarray,and then received a difference of gene expression through scanning and analysis.Result:66 genes significant differences in expression were screened out,of which up-and-down regulated gene were 20 and 46 respective.The representative genes up-regulated is FADD,CDKN2A and BID,and the down-regulated genes in is CTNNB1 and RPS3a.Conclution:1. Li Chong Sheng Sui Yin can regulate human ovarian cancer cell line SKOV3 apoptosis related genes. Activation of Fas apoptosis signaling pathway, blocking cell cycle,promoting mitochondrial release of cytochrome C,affecting cell adhesion,and inhibition of tumor cell protein synthesis, which maybe the mechanism of apoptosis.2. Li Chong Sheng Sui Yin by up-regulating FADD,CDKN2A,BID gene and down-regulating CTNNB1,RPS3a gene expression of ovarian cancer,play an anti-cancer, induce apoptosis,inhibit metastasis, improve immune function and increased sensitivity to the role of chemotherapy.