| Taihe black-bone silky fowl (TBSF) is an acknowledged breed of natural medical chicken in Chinese tradition, grows primarily in South China, Taihe, Jiangxi Province. As a traditional Chinese medicine, it can be used independently or in compound prescription, BSF has been proved to have the effects of nourishing liver and kidney, profiting vitality, reducing asthenia heat, curing abdominal pain and so on. At present, the bioactive research of the peptides, which is acquired from the meat of TBSF by enzymolysis, is still reported infrequently. It is worth of an all-sided experiment research, to find out whether there is any relationship between the TBSF peptides and the medicinal mechanism of TBSF. In consideration of aforementioned, this paper established several animal modes to study on the hemopoietic effect, anti-inflammatory effect, analgesic effect and liver protective effect of TBSF peptides. Now the main research content is summarized as follows:1. To observe the hematopoiesis activity of the TBSF peptide and one of its fractions (A), blood loss combined with myelosuppression drug (Fluorouracil,5-FU) were used to construct the blood deficiency mice model, and the red blood cells (RBC) and hemoglobin (HGB) levels were measured. Results showed that, on the 12th day, the RBC level of the bioactive peptides group has a significant difference (P<0.05) compared with the blood deficiency group; During the period of 6 to 12 days, the effect of rising the RBC and HGB level of the bioactive peptides group is significantly quicker than the Ejiao group (P<0.05). On the 18th day, the HGB level of the bioactive peptides group exceeds the other groups, and is significantly different (P<0.05) from the normal control group. The RBC level of the bioactive peptides component A group is higher than the blood deficiency group, without significant difference (P>0.05), while the blood deficiency group and the Ejiao group are significantly different (P<0.01) from the normal control group. On the 20th day, there is no significant difference (P>0.05) between the bioactive peptides component A group and the normal control group on the level of HGB, while the blood deficiency group and the Ejiao group are significantly different (P<0.01) from the normal control group.2. To observe the anti-inflammatory activity of the TBSF peptide, the carrageenan-induced paw edema in mice model, ear xylene cause inflammation model and capillary permeability model were used in the research. The result showed that, the peptides of TBSF could inhibit the synthesis or releases of PGE2 in body tissues significantly, but to the apparent edema of body, no significant inhibit effect was found. Those suggest that the peptides of TBSF exhibited anti-inflammatory activities to some extent.3. Investigations on the analgesic effects were conducted, including hot-plate test model and acetic acid-induced writhing test model. The results showed that the low and high doses of TBSF peptides did not increase the latency time of mice significantly, only a weak effect of analgesic was showed, but the peptides could significantly decreased the number of writhing, and inhibited the writhing response by 68.4%, showed their analgesic effects to chemical injury. To the hot-plate test model, the analgesic effect was not so significant, a low analgesic tendency exhibited. Those suggest that TBSF peptide has a better analgesic effect to chemical injury, rather than the thermal pain stimulus.4. To study on the liver protective effect of TBSF peptides, CCI4 and D-galactosamine induced hepatic injury model were established respectively. The results were found that the ALT, AST, MDA, LI, and SI indexes were not significantly inhibited, and the SOD level was not increased. Those means remarkable protective effect was not showed to the liver injury, the effect on the inhibition of lipid peroxidation to body tissues was inconspicuous, and peptides did not show a prominent inhibitory action to the pharmaceuticals induced liver and spleen accretion. |
PDF Full Text Request