Study On The Effect Of Transcatheter Arterial Embolization Combining Curcumin Tumor Growth And VEGF In Rabbit VX-2 Liver Tumor

Objective:The way of tumor vessels embolismed by iodinate oil can enhance the express of vascular endothelial growth factor (VEGF) protein in residual tumor cell;the experient associated curcumin with embolizer in order to observe the effect on the expression of tumor growth and VEGF in rabbit VX-2 liver tumor transcatheter arterial embolization(TAE) combining curcumin; probing the curcumin was or was not able to inhabit the growth of tumor and the enhancement of VEGFprotein in residual tumor brought out by iodinate oil。Methods:VX-2 carcinoma pieces were surgically implanted into the left liver lopes of 40 New Zealand white rabbitsin order to build the model of liver cancer,those of thirty-six were successfully transcathetered arterial embolization.those were randomly divided into the four groups,with nine rabbits in each group. Controlgroup:treated with heparin saline; B group:treated with hepatic arterial infusion therapy combining curcumin;C group:Hepatic artery embolism combining iodized oil; D group:treated with transcatheter arterial emboli-zation combining curcumin and iodized oil. Two weeks after embolism, the rabbits were sacrificed and the tumors were taken out. The volume of tumor and the rate of tumor grate was calculated before and after TAE. Immunohistochemistry and Western blotting were used to detect expression of vascular endothelial growth factor (VEGF) protein。Results:1. Variation of tumor volume A group:18.30±0.61; B group:18.36±1.11; C group:6.06±1.13;D group:5.96±0.92.draw a parallel between the two:the volume of B group was not supressed and comparedwith the volume of A group,it had not obvious differences(P=0.88);the volume of C group was supressed and comparedwith the volume of A group, it had obvious differences(P<0.01); the volume of D group was not supressed and comparedwith the volume of A group, it had obvious differences(P=0.175); the volume of C group was supressed and comparedwith the volume of B group, it had obvious differences(P<0.01); the volume of D group was supressed and comparedwith the volume of B group, it had obvious differences (P<0.01); the volume of D group was not supressed and comparedwith the volume of C group, it had not obvious differences(P=0.831).2.VEGF protein was detected by Immunohistochemistry as follow:A group 0.599±0.182, B group:0.545±0.284, C group:0.867±0.210, D group:0.603±0.186; draw a parallel between the two:the volume of B group was not supressed and comparedwith the volume of A group,it had not obvious differences(P=0.46);the volume of C group was supressed and comparedwith the volume of A group, it had obvious differences(P<0.01); the volume of D group was not supressed and comparedwith the volume of A group, it had obvious differences(P=0.592); the volume of C group was supressed and comparedwith the volume of B group, it had obvious differences(P<0.01); the volume of D group was supressed and comparedwith the volume of B group, it had obvious differences(P:0.242); the volume of D group was not supressed and comparedwith the volume of C group, it had not obvious differences(P=0.01).The trend of VEGF protein expression was as the same as that of VEGF he relative light intensityConclusions:1.Sing-use curcumin neither inhibit effectively the grate of tumor,nordepress the expression of VEGF protein inresidual tumor cell.2.curcumin epress the enhancement expression of VEGF protein inresidual tumor cell brought out by iodinate oil.,but curcumin can not obviously inhibit the grate of tumor.