Role Of Endogenous Carbon Monoxide In Whole Hepatic Ischemic Reperfusion Injury In Rats

Objective:By applying to hemin and ZnPP to study the effect of endogenous carbon monoxide on whole hepatic ischemia reperfusion injury in rats and to discuss the mechanism, in order to produce the theoretical proof on treatment of some hepatic ischemia diseases.Methods:There are 128 wistar rats, of which 8 rats were not treated except opened abdominal cavity as controll group. The other 120 wistar rats were divided into three groups:salines group, hemin group and ZnPP group. Models of hepatic ischemia was made and different drugs injected. The animals were executed after different reperfusion time. the contents of CO, NO and ET were determined in serum. Parts of the livers were put into formaldehyde liguor for fixing. The livers of the 128 rats have been studied by immunohistochemical methods to observe the expression of bcl-2, Tunnel methods were used to detect apoptosis of hepatic cells.Result:(1) The change of CO in serum:In the group of saline, the content of endogenous carbon monoxide became higher at 2 hour, and at 16 hour achieved the highest, then was down slowly. They were higher then control group (p<0.05). In hemin group, CO also increased at 2 hour, compared with the control and saline group, the increase was obvious (p<0.05). At 16 hour it achieved the peak value, and at 32 hour was down slowly. In ZnPP group, the content of CO had been down at different hours, at 16hour achieved the lowest, then rised slowly. They were lover then saline group (p<0.05). But compared with the control group, at 2,4,32 hours it was higher, at 8 hour no differencewere found and at 16 hour lower (p<0.05). (2) The change of NO and ET in serum:In saline group, the content of NO had been down at different time of reperfusion, at 8 hour achieved the least. Compared with the control, the variety was obvious (p<0.05). In hemin group, the content of NO were all higher at different time of reperfusion, at 16 hour achieved the least. The variety of ET just opposited to the NO. They had distinct to the control and saline groups (p<0.05). In ZnPP group, the content of NO became higher and ET lower, they were all achieved peak value at 16 hour, also different with the control and saline groups (p<0.05). (3) The change of bcl-2 protein and apoptosis:In saline group, the expression of bcl-2 protein was lover and apoptosis was higher, at 16 hour reached peak value, compared with the control, the variety was obvious (p<0.05). In hemin group, bcl-2 protein increased and apoptosis decreased at different time of reperfusion, they all reached peak value at 16 hour, and higher than those of bcl-2 protein, but lower than those of apoptosis. They had distinction to the control and saline groups (p<0.05). In ZnPP group, the change of bcl-2 and apoptosis were contrary to the hemin group.Conclusion:The endogenous carbon monoxide has an effect of protection for hepatic cells in whole hepatic ischemia reperfusion injury in rats.