Promotion Of Wound Healing By Bioactive Glass Material In Susscrota Domestica

Background: Bioactive glass is a kind of hyaline biological material with superior capacity, biocompatibility and workability, which is composed of oxides containing Na+, Ca2+, Si2+ and P3+. Since it was invented by Dr. Larry·Hench from University of Florida, the bioactive glass is widely used in osteology, ophthalmology and otorhinolaryngology, stomatology and so on. Under some conditions like natural disaster, trauma, burn and chronic ulcer, the exposed skins are easy to form defect or loss of function. The statistic data demonstrates that more than four million people in China with skin defect need clinical strategies each year. In this context, research in role of bioactive glass material on promoting wound healing is becoming a hot spot area. The present project is focusing on a novel bioactive glass and its effect on promoting full-thick skin defect, and make groundwork for its further utilization in skin wound healing.Objective: To observe the facilitative function of wound healing through sprinkling bioactive glass material in Susscrota domestica.Method: Fourteen pig models of skin wounds with loss of their full-thickness layers (each pig with six wounds of 4 cm×4 cm) were established for the observation of the effects on the wound healing. The biological active glass materials were fully sprinkled into the skin defects and the healing time were recorded each day. On day 1, 3, 7, 14, 21, 28, 35, took photos and calculated the healing rate. Meanwhile, a small tissue was excised from the central part of each skin wound, fixed in formalin, embedded in paraffin, and cut into 4μm for HE staining. The data was analysed using spss16.0 software.Results: The healing times of experimental and blank group were 23.19±1.27d and 29.52±1.54d, respectively (P < 0.05). The healing rate was significantly higher and the healing time was significantly shorter in the experimental group than in the control group (P < 0.05). The histological examination showed that the extent of epithelialization condition and epidermal growth was also much better in the experimental group than that in the control group. The amounts of fibroblast and capillary were much more in the experimental group than that in the control group.Conclusion: The bioactive glass material may be considered as a prospective method in promoting the healing of wound with a loss of full-thickness skin.