Recurrent Pregnancy Loss And Sperm Quality And Form Abnormal Discuss The Relation

Background and purposeRecurrent pregnancy loss is obstetrics and gynaecology common diseases,for all the pregnancy rates about 2% to 5%. Patients often experience multiple pregnancy and abortion, Some patients are as high as 8-9 times miscarriage. On their health and cause greatly affect the anxiety, triggering emotions and during pregnancy again fears, Serious when the bursting of the lead to marriage and family feud, therefore the etiology for RPL research has important theoretical significance and practical value.Research shows that recurrent pregnancy loss pathogenesis is extremely complex, except with heredity, anatomy, reproductive endocrine, infection factors, such as the outside, still have part of the etiology and pathogenesis is unknown. At present the etiology of recurrent pregnancy loss repeatedly research focuses on women, that the main factors associated with immune factors as providing embryonic half the men less genetic factors. Embryos is after fertilization by eggs and sperm development and become, man gametes constitute half of embryonic genes, so men of miscarriage factors on having the effect that cannot ignore.About sperm itself factors and miscarriage, current research mainly involves sperm quality, morphology, sperm DNA and so on, but there is no consensus conclusions. Sperm quality mainly through conventional semen analysis understand sperm quantity, sperm density, and semen of the total number of sperm, sperms agglutinate, sperm motility and live rate, sperm forms, semen liquefaction and other information. Sperm forms is an important index evaluation male fertility, research shows that no matter in conventional semen analysis, in vitro fertilisation embryo transplantation and single sperm injection in the cytoplasm is predicting success rate fertilization in sperm forms analysis is an important parameter index. In order to further research sperm quality and form to RPL influence, this paper mainly through repeated natural abortion patients for husband and normal birth men routine analysis and semen analysis detection, sperm morphology and compare the difference between two groups of numerical miscarriage for diagnosis, the etiology of up to provide theoretical foundation, RPL part of clinical diagnosis, prevention, treatment and assisted reproduction treatment is of great significance.Materials and methods1 SubjectsIn January 2005 in October 2010 in zhengzhou university between October 1st affiliated hospital maternity and reproductive medical center of spontaneous abortion doctor repeatedly in couples aged 22 to choose between 40,Somatic chromosome abnormality exclude both sides of husband and wife, endocrine and reproductive system anatomical abnormal, immunology, diabetes, hypertension, genital infection known abortion of related factors of patients couples, as the research object 62 cases as a team to take patients husband semen. Meanwhile randomly given birth of 40 male patients with healthy children. Requirements for the control group semen age between the two groups, and no significant difference of selected case data integrity.2 Research methods2.1 Specimen collection Sex thought for 4-7 days in take fine room masturbation method in the disinfection of take dry clean jar, on the inside of the tub after completely liquefaction metastasize to measure volume, centrifugal tube calibration just referred.2.2 Operation method According to WHO's operation method, analyzed semen liquefaction amount of time, pH value, viscosity and sperm forms, etc. First, the semen liquefaction completely take a drop of about 10 sperm drops on the slides, thin, with improved pap smears dyeing method dyeing inspected.2.3 Semen speciation analysis Refer to the world health organisation standards, to adopt domestic WLJY - 9,000 type color sperm forms detection system of artificial modification methods has been carried out under the sperm forms analysis. Each sample analysis 100 sperm, computation form normal sperm rate.2.4 Inspection index Sperm quantity, pH value, liquefied time, sperm density, sperm live rate, sperm motility, and normal sperm forms. Semen morphological index: will the sperm into normal sperm and abnormal sperm, the abnormal sperm and two types according to the position of head different cent deformity anomalies and body of the abnormal, tails anomalies and mixed abnormalities.3 Statistical methodsAccording to statistics, ask to handle each parameter SPSS 17.0 statistical software using statistical analysis, quantitative material are to say, compares the quantitative data set, between t test between qualitative data set compares the inspection, P< 0.05 said a statistically significant difference.Result1 Routine examination:control the semen 2.48 amount of semen pH ml, plus or minus 1.07 for 7.35 + 0.21, liquefaction 28.33 + 7.97 s, time for 50.46 + sperm density 15.54 x 106/ml,60.34 + 16.32% sperm live rate, sperm motility (a + b level) 43.19 + 19.77%, normal sperm rate 70.12 + irrigated land. Sperm quantity group for 2.61 + 1.20 ml, pH value for July 28 + 0.76, liquefaction 27.56 + 7.21 s, time for 48.67 + sperm density 17.23 x 106/ml,42.71 + 18.38% sperm live rate, sperm motility (a + b level) 38.33 + 22.09%, normal sperm rate 10.21% 32.40 +. The result shows:the two groups of semen quantity, pH value, liquefied time, the difference between the sperm density was not statistically significant (P>0.05). Compared with control, the team sperm live rate, sperm motility, normal sperm rates were significantly lower, a statistically significant difference (P<0.05).2 sperm forms analysis:The control group 8.37% 32.07 + normal form, abnormal sperm, the head 67.93 + 8.37% sperm deformity 33.05 + 9.09%, body of the deformity 7.45 + 6.12%, tail 10.38 + 5.28%, mixed deformity 14.01 + this age was 8.32% deformity. Research 13.20%25.48 + normal form, abnormal sperm, the head 74.52 + 13.20% sperm deformity 33.99 + 13.49%, body of the deformity 7.45 + 5.84%, tail 21.88 + 4.62%, mixed deformity 14.24 + 8.15% deformity. The result shows:the two groups of sperm deformity speciation analysis in the head and body of the deformity sperm percentage sperm percentage and tail teratozoospermia plus the difference between the percentage was not statistically significant (P> 0.05). Compared with control, the team's abnormal shape sperm deformity, tails percentage were significantly increased sperm percentage rate, and normal sperm forms is decreased obviously, the percentage are statistically significant difference (P< 0.05)Conclusion1 repeated natural abortion were male sperm live rate to decrease, sperm motility is reduced, the normal form sperm decrease.2 repeated natural abortion were husband sperm forms normal form in examining sperm reduce multiplied, the tail, deformity sperm deformity sperm significantly higher percentage. Therefore, in the treatment of repeated natural abort, can improve sperm quality or lines sperm separation separate normal sperm intraoperative assisted reproductive technologies, followed up to the purpose of miscarriage treatment.