| Wheezing is a common symptom of children under 5 years of age, because of the unique aspects of the preschool-aged children as well as the physiologic and pathologic features of the disease, wheezing often occur more than once. Parts of wheezing is the early onset of asthma. Thus, discriminating from the wheezing children under five years old who may develop into persistent asthma and taking early useful intervention are very necessary,but not all the wheezing children could develop into asthma,so there are not enough markers which we could depend on to choice the subjects for intervention.PurposeThis clinical study attempts to explore the correlation between wheezing children under five years old and asthma in molecule level and provide the molecular biology evidence for asthma early diagnosis through analyzing genotypes and alleles frequency of TARC promoter -431 loci in reccurent wheezing children under five years old, and the characteristic of the plasma concentration of TARC,total IgE and the peripheral blood eosinophil count (PBEC) in each genotypes.Thus,we could study the relationship between polymorphism in TARC gene and the pathogenesis of childhood wheezing.Materials and methodsThis experiment is divided into wheezing group(for 118 cases) and the control group(for 40 cases). Subjects in wheezing group are all from Respiratory out-patient clinc and hospitalized patients from the October 2009 year-May 2010, the numbers of wheezing> 2 times in six months or≥3 times in 12 months was called recurrent wheezing. According if the children have a doctor's diagnosis of allergic diseases (including eczema, allergic dermatitis, allergic rhinitis or food allergy), or either/both of their parents have a history of asthma risk factors for atopic quality standards, wheezing group were divided into wheezing group I (with one risk factor)(for 70 cases), and wheezing groupⅡ(no risk factors for the children)(for 48 cases). And the random sample of preoperative 40 cases are chosed as a control group from children who has kidney stones and other non-infectious diseases over the same period in the hospital and surgical hernia.All inrolled children were full-term births, and they all had no history of systemic and local hormone application in two weeks.There was no significant difference in ageand gender (P> 0.05) between three groups.The genotypes and alleles frequency of TARC-431 loci in wheezing group I, wheezing groupⅡand control group were detected with PCR-RFLP analysis. The frequency of genotype and loci of TARC-431 in three groups were compared.The plasma concentration of TARC, total IgE and PBEC were detected with enzyme-linked immonosorbent assay(ELISA).The PBEC count was determined with eosin staining method.The TARC, total IgE and PBEC in three groups and the TARC, total IgE and PBEC of each genotype were compared.Results1. TARC promoter -431C-T gene polymorphism was present in each group.There were three kinds of genotypes of the gene polymorphism.The frequency of each genotype of wheezing group I was CC,CT,TT(31.4%,5.7%,12.9%) respectively,and the frequency of each allele was T,C(40.7%,59.3%);the frequency of each genotype of wheezing groupⅡwas C,CT,TT(35.4%,54.2%,10.4%) respectively,and the frequency of each allele was T,C(37.5%,62.5%);the frequency of each genotype of control group was CC,CT,TT(37.5%,52.5%,10.0%) respectively,and the frequency of each allele was T,C(42%,58%).There was no significant difference in each group(P>0.05) for the genotype and allele frequency.2. The serum levels of TARC and PBEC in groupⅠwere higher than the groupⅡ(P<0.01)and the control group (P<0.01),and the levels of TARC and PBEC between the groupⅡand the control group have no difference(P>0.05);the levels of TIgE in groupⅠand groupⅡwere higher than the control group (P <0.01),while there is no difference between the groupⅠand groupⅡ(P>0.05); serum TARC and PBEC have positive correlation (r=0.872,P<0.01).3. The serum TARC level of TT genotype was signifcant higher than CT genotype (Z=-3.209, P<0.01) and CC genotype(Z=-3.656, P<0.01) in groupⅠ,and there was no difference among the three genotypes in groupⅡand control group (H=0.274,P>0.05;H=2.177,P>0.05).4. The serum TIgE level of TT genotype was signifcant higher than CT genotype (Z=-4.043, P<0.01) and CC genotype(Z=-3.278, P<0.01) in groupⅠ,and there was no difference among the three genotypes in groupⅡand control group (H=0.846, P>0.05;H=1.934,P>0.05).5. The serum PBEC level of TT genotype was signifcant higher than CT genotype (Z=-2.841, P<0.01) and CC genotype(Z=-3.287, P<0.01) in groupⅠ,and there was no difference among the three genotypes in groupⅡand control group (H=5.431, P>0.05;H=3.943,P>0.05).ConclusionsTARC promote -431 loci genetic polymorphism is not correlated with recurrent wheezing which often occured in children under 5 years old,but it is correlated with atopy, the serum of TIgE and PBEC... |
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