Bacterial Resistance Detection And Analysis Of Trands Of Drug Resistance From Samples Of Clinically Isolated

Objective:To investigate the Bacterial distribution and antibiotic resistance isolated from the clinical patients in the Second Hospital of Shandong University during the year 2009-2010,and to provide a reasonable choice for rational use of antibiotics in clinical practice.Methods:Distribution and drug resistance of a total 3259 (in year 2009)&4105 (in year 2010) non-duplicate isolated specimens in the Second Hospital of Shandong University in 2009-2010 were analyzed. The VITEK identification system was used for bacterial identification, the drugs sensitivity testing was investigated with the disk diffusion method (KB method),the data statistical analysis was detected by the microbiological testing Chinese system ATBPLUS Ver3.3.Results:The isolates were mainly Gram-negative bacteria (accounting for 56.61% in year 2009& 57.25% in year 2010). The resistance rates of Escherichia coli to penicillins such as piperacillin, ampicillin were 78%,92% respectively in 2009,however,sensitive to the carbapenem. Klebsiella pneumoniae was in general resistance to penicillin, cephalosporins, aminoglycosides and other antibiotics, but no resistance to imipenem. Pseudomonas aeruginosa resistance rates to imipenem and meropenem were 30% and 33% in 2009, but the peptide antibiotic polymyxin was 1% in 2009. The resistance rates of Staphylococcus aureus to penicillins, cephalosporins, macrolides, lincomycin and other classes of antibiotics were all above 80% in year 2009 & 2010, but found no vancomycin resistant strains.Conclusions:Bacterial resistance to antibiotics in our hospital are more serious, reasonable choice of antibiotics and strictly sterile system, strict control of opportunistic infections and nosocomial infections should be strengthened.