Functional Improvement Of Infarct Heart By Implantation Of Human Adipose-derived Mesenchymal Stem Cell Deliverd By Matrigal

The heart is one of the least regenerative organs in the body and, consequently, loss of myocardium to infarction or other diseases often leads to heart failure[1-4]. Stem cells offer the possibility of repairing damaged organs like the heart from their component parts, and there is an intensive effort to develop stem cell-based strategies for cardiac repair. Cardiomyocytes derived from human adipose-derived mesenchymal stem cells (ADMSCs) potentially offer large numbers of cells to facilitate repair of the infarcted heart. However, this approach has been limited by inefficient differentiation of ADMSCs into cardiomyocytes, insufficient purity of cardiomyocyte preparations and poor survival of ADMSCs derived myocytes after transplantation.Extracellular matrix (ECM), which plays important structural and biological roles in growth and develoment, is the microenvironment for cells to survive. It is also the essential component element of tissues and organs. Basement membrane matrix is a kind of extracellular matrix complex extracted from the EHS mouse sarcoma cells which contains laminin,collagen I,heparinoid,proteoglycan,growth factor, Matrix Metalloproteinases (MMPs) and other substances. Matrigel can play a key role in the process of cell proliferation, differentiation, migration[5-6]. The study is to investigate the ability of Matrigel combined with human adipose-derived mesenchymal stem cells to enhance the cell survival and improve the ventricular function of infarcted heart after injected into the infarcted rat heart.In this study, human adipose-derived mesenchymal stem cells (ADMSCs) were isolated and cultured from adult adipose tissue. SD rats with one-week-old myocardial infarction were randomly divided into four groups:PBS, Matrigel, PBS+ADMSCs, Matrigel+ADMSCs. ADMSCs were injected into border area of ischemia ether in Matrigel or saline. Controls received Matrigel or saline injection. At 4 weeks, heart function was determined by using echocardiography. Microvessel densities within the infarct area were also measured. This study found that after 4 weeks of implantation, the heart functions and the microvessel densities within the infarct area improved in the Matrigel+ADMSCs group compared with other groups. The co-injection of ADMSCs with Matrigel enhances grafts survival, increases microvessel density in myocardium and contributes to cardiac function.