| Background:Stem cell transplantation is a treatment of myocardial infarction which is a very promising new approach. Transplanted mesenchymal stem cells can differentiate into myocardial cells and vascular endothelial cells or secrete vascular growth factors, promote myocardial angiogenesis, improve heart function, under the certain microenvironment.Therefore, people try to interfere the stem cells using those genes which are advantageous to the survival and adhesion of stem cells or promote angiogenesis, or to modify the stem cells with certain mesures to improve the survival and differentiation of stem cells directly and indirectly in the ischemic myocardium microenvironment, and to improve cardiac function and repair necrotic myocardium by help to promote the transplanted stem cells.Objective:To compare the influences of ADMSCs transplantation treated and non-treated with bFGF on angiogenesis of infracted myocardium in rat, and to provide experimental basis for clinical treatment of myocardial infarction.Method:1. ADMSCs of mouse were isolated, cultured and identified, and the third generation of cells was used in the experiment. Forty five healthy adult SD rats were selected and the myocardial infarction model was established. Then they were divided into three groups randomly, Group PBS, Group ADMSCs, Group bFGF+ADMSCs15in each group, and5in control group,.2. To establish the model of myocardial infarction by ligation of the left anterior descending branch in SD rats.3. PBS and two kinds of stem cells labeled with DAPI were injected directly into the myocardium of model rats, and then to compare the cardiac function, myocardial differentiation and angiogenesis of rats in each group4weeks later.Results:1. The LVEF of the rats with MI reduced in all the groups (P<0.05); The cardiac function of group bFGF+ADMSCs(1W:59.71±4.56,4W:64.88±3.76) was significantly better than that in group ADMSCs(1W:53.40±3.43,4W:58.42±2.07) and group PBS(1W:47.03±2.11,4W:49.53±1.32)1and4weeks later(P<0.05), and the cardiac function in the group PBS was the worst(figure2).2. Immunofluorescent staining showed that both DAPI and cTNT antibodies positive cells were found4weeks later after transplantation of bGFG+ADMSCs and ADMSCs.3. The vascular density in the rim of myocardial infarcted area in each group was detected by the end of fourth week using factor VIII IHC to compare the differences between groups. Statistical results showed that vascular density of MI area in group PBS was50.34±5.58/mm2; in Group ADMSCs was103.45±4.32/mm2and in Group bFGF+ADMSCs was140.38±6.54/mm2, respectively. The vascular density in Group ADMSCs and group bFGF+ADMSCs was significantly more than that in group PBS(p<0.01); and that in group bFGF+ADMSCs was significantly more than that in group ADMSCs(p<0.05).4. Western blot showed that the VECF expression in Group ADMSCs(1.21) and group bFGF+ADMSCs(1.45) was significantly more than that in group PBS(0.79), and that in group bFGF+ADMSCs was the highest.Conclusion:1. bFGF combined with ADMSCs transplantation treating MI in rats can make the stem cells differentiate into cardiomyocytes, which can better the cardiac function and enhance the angiogenesis. 2. bFGF+ADMSCs can significantly improve cardiac function, promote angiogenesis, which is better than of simple stem cell transplantation. |
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