| PartⅠSATB1 is an independent prognostic marker for gastric cancer in a Chinese populationObjective: To investigate the prognostic significance of SATB1 expression in the development and progression of gastric cancer.Methods: We used immunohistochemical staining, western blotting and qRT-PCR to examine the expression of SATB1 in 118 cases of gastric cancer. The correlations between SATB1 expression and clinicopathological factors were statistically analyzed.Results: SATB1 mRNA expression was significantly up-regulated in gastric cancer specimens as compared with that in normal tissues (P<0.001). Over-expression of SATB1 protein was observed in gastric cancer cell lines by western blotting. SATB1 expression was detected in 44.9% (53 of 118) of the gastric cancer specimens by immunohisto- chemistry. The expression of SATB1 mRNA agreed well with the western blotting and immunohistochemical findings (P<0.001). SATB1 expression was positively correlated with age, depth of invasion, lymph node metastasis, distant metastasis and higher TNM stage. Kaplan-Meier analysis revealed that SATB1 over-expression was associated with a significantly worse survival (P=0.001). Further multivariable analysis indicated that high SATB1 expression was an independent prognostic indicator for gastric cancer (P=0.023).Conclusion: Over-expression of SATB1 was correlated with metastatic potential of human gastric cancer and would be a novel independent prognostic marker for predicting the outcome of gastric cancer. PartⅡExpression of SATB1 and heparanase in gastric cancer and its relationship to clinicopathologic featuresObjective: To investigate the expression of SATB1 and heparanase in human gastric cancer as well as their relationship with the clinicopathological factors.Methods: Specimens from 102 patients who underwent radical gastrectomy between 2000 and 2002 were studied by immunohistochemistry for SATB1 and heparanase expression.Results: SATB1 and heparanase were positively expressed in 48.0% and 51.0% of gastric cancer cases, respectively. The expression of SATB1 and heparanase was significantly correlated with depth of invasion, TNM stage, lymph node metastasis, while SATB1 expression was also significantly correlated with distant metastasis. Patients with SATB1-negative expression and heparanase-negative expression had higher survival rates than those with SATB1-positive or heparanase-positive expression. Moreover, a positive correlation was found between SATB1 and heparanase. In multivariable analysis, SATB1 expression was also identified as an independent prognostic indicator for gastric cancer.Conclusion: Our results suggested that combined analysis of SATB1 and heparanase expression may have significant value in determining invasion and metastasis of gastric cancer and assessing prognosis in patients with gastric cancer. |
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