Research On Continuous Glucose Detection In Interstitial Fluid Based On Microdialysis

Blood glucose detection plays a key role in diagnostics and treatment for diabetes. The traditional method is not only painful, but also unaccessible to the continuous result. The non-invasive method depends on the interaction between light and specific tissues of human body, with serious individual differences and difficulty in signals extraction. The minimally-invasive method, as a hot research, detects the glucose concentration in interstitial fluid to predict the of the blood glucose level, being able to implement continuous and dynamic glucose detection. However, due to the influence of the physiological activities of human body, there is a delay between the concentration of the glucose in the interstitial fluid and in blood. In order to explore the delay, physiological delay parameters between the two concentrations have to be brought in, a delay model needs to be built, and the experimental verification by accurate detection of the glucose concentration both in interstitial fluid and glucose is necessary for the model. But, it is still a world's problem that how to get the exact value of the glucose concentration in interstitial fluid continuously.In this paper, a continuous detection method of glucose concentration in interstitial fluid based on microdialysis was explored. A system based on this was built. In vitro detection experiments by simulation of continuous changing in glucose concentration and in vivo experiments in animal models were carried out. The effects of flow rate, concentration and temperature on the recovery were explored, and the accuracy of the continuous detection of glucose concentration in interstitial fluid based on microdialysis was verified by ultrafiltration. The following works are accomplished:(1)The method of continuous detection in the interstitial fluid based on microdialysis was researched theoretically. The mathematical model of recovery of the microdialysis was analysised, and the method of recovery measurement is discussed.(2)A system for continuous detection of glucose concentration based on microdialysis was built. Glucose concentration detection based on microdialysis was researched by glucose solution. The effects of flow rate, concentration and temperature on the recovery were explored. (3)A platform that simulates the continuous changing of glucose concentration in interstitial fluid was built. Such research laid the foundation for validating the technique and equipment for detection of glucose concentration based on microdialysis.(4)A detection method of glucose in interstitial fluid based on ultrafiltration was researched. The effects of vacuum, length of the probe membrane, and length of the connecting tube on the extraction rate were explored. These studies laid the foundation of animal experiment.(5)Experiments of microdialysis and ultrafiltration in animal models were carried out. In vivo microdialysis recovery measurement was researched. On these bases, the precision of microdialysis was verified by ultrafiltration. All these studies laid the foundation for the research of delay model between the glucose concentrations in the interstitial fluid and in blood.