Study On The Properties Of Chitosan/Oxidized Regenerated Cellulose Composite Hemostatic Gauze

Chitosan(CTS) has a variety of physiological activities, include hemostasis, antimicrobial activity, stimulation of healing. Chitosan is also a biodegradable material that has biodegradable, biocompatible, non-antigenic, anti-inflammatory and analgesic activities. In this paper, a composite of Chitosan/Oxidized regenerated cellulose(ORC) was prepared by using ORC grafted by chitosan with different molecular weights and we tested its ability to rapidly hemostatic properties, kill bacteria in vitro and the degradation.In this paper, the C6 oxidized regenerated cellulose with different–COOH content were graftded by chitosan with three different molecular weights(Mw). Chitosan with the lowest Mw of 2,000 introduced on oxidized regenerated cellulose surface was highest and increased with oxidation time, while chitosans with medium and high Mw showed that the maximum percentage of chitosan introduced on oxidized regenerated cellulose surface occurred at the oxidation time of 8h. The characteristics of FT-IR and DTG suggested the formation of the amide bond between the chitosan and the oxidized regenerated cellulose. Chitosan/oxidized regenerated cellulose composite hemostatic gauze neutralized by NaOH/ethanol solution was demonstrated water-soluble. FT-IR showed that -COONa group was formed. Further observed by the SEM , the surface of neutralized fiber displayed many cracks.We tested its ability to rapidly hemostatic in Rabbit model of liver. Before the neutralized, the CTS2/ORC that the best composite of the hemostatic gauze, the average time was 255s, the average blood loss was 0.54g, less 20s than ORC. After the neutralized, the CTS3/ORC-Na that the best composite of hemostatic gauze, the average bleeding time was 145s, the average blood loss was 0.25g, is less 130s than ORC. The high Mw chitosan could increased the hemostatic effect of oxidation regenerated cellulose, the type of water-soluble hemostatic gauze is better than non-soluble hemostatic gauze. In Rabbit ear artery model, the data suggest that the best composite hemostatic gauze is CTS1/ORC, the average time was 155s. The antimicrobial ratio of oxidation regenerated cellulose against Staphylococcus aureus and Escherichia coli is 99.9%. We found that the non-water-soluble hemostatic gauze need 14 to 21 days can complete be degraded, water-soluble compound hemostatic gauze need 7 to 14 days can be almost completely degraded in vivodegradation. The degradation rate of Chitosan/oxidized regenerated cellulose composite hemostatic gauze is also affected by the molecular weight of chitosan. Chitosan with a molecular weight of 2000, is the fastest degradation of the composite hemostatic gauze, and all the complex hemostatic gauze safe, with well biocompatibility, did not cause inflammatory reactions.