The Study On Mechanism And Interference Of Auricularia Auricular Powders On Silicosis Fibrosis In Rats

Objective 1.To establishthe silica-induced pulmonary fibrosis model in rats; 2.To observethe influence of Auricularia auricular powders (AAP) onthe silicosis fibrosis in rats; 3.To explorethe mechanism ofthe effects of model rats bythe histopathology,the activity ofthe SOD,the contents of MDA,NO and HYP. Methods Eighty rats were randomly divided into control group, silica control group, and AAP -treated groupⅢ,ⅡandⅠ. Silicotic control group, AAP -treated groupⅢ,ⅡandⅠanimal models were established by direct tracheal instillation of silica into rat lungs non-surgically. After modeling 24 hours,rats in AAP-treated groupⅢ,ⅡandⅠwere given AAP by feeding. At modeling 14^th,56^th days establishment ofthe animal model, eight rats were killed in a group at a time and calculate lung/body coefficient. Lung fibrosis in morphology was observed by H.E staining,and collagen content was detected by Hydroxyproline assy. Quantity analysis of fibrosis content by Sirius Red staining and Image analysis system.The superoxide dismutase (SOD) activities, maleic dialdehyde(MDA)content and glutathione peroxidase (NO)content were assayed by colorimetry method. Results⑴the changes of lung/body coefficient: At 14th day,lung/body coefficient in AAP-treated groups were significantly higherthanthose of SiO₂ control group(P<0.05) . lung/body coefficient in AAP-treated groupⅢwere still significantly lowerthanthose of SiO₂ control group(P<0.05). At 56^th day,lung/body coefficient in AAP-treated groupⅢwere still significantly lowerthanthose of SiO₂ control group(P<0.01).⑵Pathologic inspection:At 14^th , The Pathologic injury degree of 87.5percent rats lung in AAP-treatedⅢgroups and 25.5percent rats lung in AAP-treatedⅡgroups was lighter thanthat of SiO₂ control group, at 56^th day,62.5 percent rats lung in AAP-treatedⅢgroups was lighterthanthat of SiO₂ control group,and lighter l/2 Pathologic grade.⑶the changes of fibrosis content: At 14^th ,56^th day, The fibortic area percentage of rats lung was lower in AAP-treated groups different degreely^thanthose of SiO₂ control group, AAP-treated groupⅢwas lowerthanthose of SiO₂ control group(P<0.05).⑷the changes of HYP content:At 14^th day,HYP content in AAP-treated groups was lowerthanthose of SiO₂ group(P<0.05). HYP content in AAP-treatedⅢandⅡgroups was significantly lowerthanthose of SiO₂ group(P<0.05).At 56^th day , HYP content in AAP-treatedⅢgroup was significantly lowerthanthose of SiO₂ group(P<0.01).⑸the changes of SOD content:At 14^th day,SOD activities in AAP-treated groups was significantly higher than those of SiO₂ group(P<0.05) . At 56^th day,SOD activities in AAP -treated groupⅢwas still higher than those of SiO₂ group(P<0.05).⑹the changes of MDA conten: At 14^th day, MDA content in AAP-treatedⅢandⅡgroups were significantly lower than those of SiO₂ group(P<0.05) . At 56^th day,MDA content in AAP-treated groupⅢwas still significantly lower than those of SiO₂ group(P<0.01).⑺the changes of NO conten:At 14^th day,NO content in AAP-treated groupⅢwas lower than those of SiO₂ group(P<0.05). At 56^th day,NO content in AAP-treatedⅢgroup were lower than those of SiO₂ group(P<0.01). Conclusion AAP can inhibit synthesization of lung collagen and ameliorate the silica-induced pulmonary fibrosis by increasing the activities of SOD ,inhibitting MDA and NO content and alleviating the damage of peroxidation to the lungs.