Relationship Between Endothelial Cell Derived Microparticles And AGVHD In Allogeneic Hematopoietic Stem Cell Transplantation

Objective To investigate endothelial cell derived microparticles from peripheral blood and endothelial injury related cell factors (Ang-1, Ang-2, VEGF and TNF-α) in allogeneic hematopoietic stem cell transplantation to probe the pathogenesis of aGVHD. To prove EMPS released from HUVEC after stimulated by TNF-αin vitro.Methods In this study, 21 patients underwent allo-HSCT,①to detect EMPS which express CD62E-PE from peripheral blood by flow cytometry(FCM).②Cell factors Ang-1, Ang-2, VEGF and TNF-α,which related to endothelial injury were detected by ELISA .③EMPS from HUVEC stimulated by TNF-a (different concentration and several action times)were detected by FCM.Results①FCM method showed that the level of EMPS in aGVHD group at +21d after allo-HSCT is higher than no aGVHD group(P<0.05),the level of EMPS at aGVHD point is lower than aGVHD group at +21d(P<0.05),but there is not differernce with other point.②ELISA revealed that Ang-1 increase obviously at +7d in aGVHD group which compared to no-aGVHD group. aGVHD point group had sharply declined than other groups. Ang-2 of aGVHD group was higher than no aGVHD group at +21d, the disparation had significance(P<0.05). The level of TNF-αat +21d were significantly higher than that of aGVHD point group. The level of VEGF is Lowest at +7d than other points.③EMPS stimulated from HUVEC for 24h are more than other time point. In certain range of concentration of TNF-α(10,50,100, 150ng/ml), FCM showed that there are more EMPS than other concentration at 100ng/ml.Conclusion①The level of EMPS and Ang-2 after HSCT were higher than that before HSCT .They not only reflect endothelial injury of aGVHD but also relate with the occurrence of aGVHD.②Ang-1,TNF-αand VEGF could reflect endothelial injury after transplantation state.