The Studies Of The Effects Of Anti-CVB Myocarditis Capsule Extractive Saponins On CVB Viral Myoearditis In Mice

ObjectiveIn this study, male Balb/c mice were modeled with coxsackie B₃ virus (coxsackievirus B₃, CVB₃) through intraperitoneal injection, preparation VMC model was dealt with A-CVB-MC compound extracted saponins. Myocardial protection in mice VMC was observed,and its possible mechanism was explored,all of which are used to provide laboratory evidence for the development of new drugs.Methods1. Animal Model Selection Male Balb/c mice,being adaptive fed for a week,25 mice were randomly selected as normal controlled group (Normal control group,N),intraperitoneal injection of 0.2ml sterile undiluted Hep-2 cell lysate.The remaining mice were injected intraperitoneally sterile 0.2ml0.8ID₅₀ the CoxB₃ virus,the establishment of VMC model.Myocardi- tis mice induced by CVB₃ infection appeared emaciated,dull sparse fur,less activity,loss of appetite,tremors and a series of symptoms.Elevated serum enzyme activity and cardiac pathology index are criterions a successful model.2. Experimental program 150 mice were selected and divided into 6 groups randomly: model group,normal group,comparison group (ribavirin), high-dose-saponins group, middle-dose-saponin group,low-dose-saponin group,25 in each group.Normal control group and model group affect with distilled water,the other groups fed with the appropriate drugs were injected in each group 2 hours after gavage, daily administration once in each group, lasting for 14ds,volumes are 20ml·kg^-1. Charts are protracted focusing on macroscopic signs of form, once observed and recorded daily body weight of mice, death, fur color, shape, eating and activities such as living conditions, 8 mice in each group were taken on the 8th day, the rest were taken on the 15th day, all of which were operated for orbital blood serum enzymes and cTnI were detected. All of mice were killed by cervical dislocation,sterile removal of the heart,spleen and thymus, all of which were quickly weighed to calculate wet weight index,the heart of routine biopsy,the pathological changes of myocardial tissue.Results1.A-CVB-MC-S on the overall status of each group of mice, body weight and mortalityIn the first 3 days after model, except the normal controlled group, the other mice in each group appeared back hair messy, dull, curled up kyphosis, reduced activity of light stimulation, biting each other, and light weight,etc.The treatment group compared with the virus in mice model mice such mild symptoms, and the model group, SL, R group showed bad ears, bad legs, unfinished phenomenon, no other group in the disease.To model the experimental mice on 7th day was significantly lower than the normal weight group, treatment group status of the other little better, but no significant difference between the model groups, although the weight loss, but the treatment group occurred before mice were killed and slow weight growth trend. From the 3rd day of the experiment there were some mice were dead,the death peak occurs in the first 5-8 days,saponins can be used to reduce the dose of mortality in mice,especially in SM group best effect,each treatment group can effectively delay time of death in mice.2.A-CVB-MC-S for each group of mice heart, spleen, thymus weight index of the wetWeight index of heart, spleen, thymus were increased in each group. After administration,these indicators began to decline,particularly in SM group heart weight index and spleen weight index decreased significantly,compared with model group,significant difference(p<0.05), and thymus index of each group was no significance.Experimental data show that the three organs of heart weight index fell the most obvious.3.A-CVB-MC-S serum levels of various enzymes AST,CK,CK-MB,LDH and the activity of HBDHAfter infection,the serum AST,CK, LDH,CK-MB and HBDH content increased.Compared with model group,the treatment group decreased activity of the above indicators.Two test results showed the efficacy of various drugs favorably to the times of the order: SM>SH>R>SL group,in which SM,SH group decreased LDH,CK,CK-MB levels of significant effect,compared with model groupdifferenceP<0.05.4.A-CVB-MC-S mice in each group of cTnIControlled group, serum troponin I (cTnl) the level of traces, in the 8th day after model, serum cTnl increased significantly in model group and treatment groups,cTnl in treatment groups were lower than that of model group (P<0.05).In the 15th days, SM group compared with the M group decreased serum cTnl the most significant (P<0.01).5.A-CVB-MC-S mice in each group pathological changes of myocardialIn normal controlied group of mice myocardium,no significant pathological changes.The remaining mice in each group had varying degrees of heart disease, visual inspection under the M, R mice were like fat white surface of the heart disease,myocardial cells under light microscope arranged orderly,swelling, necrosis and extensive inflammatory cell inflammatory cell infiltration,myocardial inflammatory reaction around the small blood vessels clear and other diseases.The treated group after treatment and necrosis of myocardial pathological changes were significan- tly lighter than the model group.Pathological scores of evaluation results indicated that the model group were significantly higher than that of pathological scores of each treatment group,both were statistically significant (P <0.05).ConclusionA-CVB-MC-S can be used to improve symptoms of VMC mice significantly,the survival rates of mice on the VMC mice have an overall protective effects,which can reduce the enzyme activity in mice with myocarditis and cTnI level,reduce myocardial cell damage and reduce heart weight index, improve myocardial pathological changes and reduce the myocardial pathological score, good myocardial protection. The mechanism may be enhancing immune function, reduce the immune inflammatory response.