| The Magnolia Flos is our country's traditional traditional Chinese medicine, Plants the people's hopes for the wooden orchid aceae spring to spend Magnolia biondii Pamp., magnolia Magnolia denudata Desv. Or Magnolia sprengeri Pamp. dry flower bud. The winter end spring first flowering has not opened when recovers, except a stem, dries in the shade.The Magnolia Flos simultaneously is also our province road place raw material for medicine, besides volatile oil, but also includes the wooden fat element, the alkaloid and so on each kind of effective component, has the sterilization, the voltage dropping and so on many kinds of pharmacological actions, although at present had other scholars to formulate Magnolia Flos raw material for medicine volatile oil GC and the GC-MS fingerprint, but limits in the extraction method to the traditional steam distillation, time-consuming is long, and is big to raw material for medicine and the resolver consumption, But regarding the Magnolia Flos in the other type's effective component, through establishes its highly effective liquid phase fingerprint atlas, between the research different technology obtained fingerprint atlas's relations, may let the fingerprint true reflection Magnolia Flos the intrinsic quality. In addition, in the literature also not yet sees the Magnolia Flos to go against the spatial solid phase micro extract- gas chromatography fingerprint and the highly effective liquid chromatography fingerprint report. Therefore the research raw material for medicine Magnolia Flos goes against the spatial solid phase micro extract-gas chromatography fingerprint atlas and the highly effective liquid chromatography fingerprint, provides the method and the basis for the Magnolia Flos raw material for medicine's distinction and the quality control, has the very vital significance.This article established the Magnolia Flos to go against the spatial-solid phase micro extract-gas chromatography for the first time (HS-SPME-GC) the fingerprint, has provided one better method for system's science appraisal and the appraisal Magnolia Flos's quality. Using the HS-SPME technology directly extracted the volatile organic compounds in Magnoliae Flos, then these compounds be separated and identificated by GC and GC-MS. The Chromatographic fingerprint of Magnoliae Flos was established by HS-SPME-GC. The 47 common peaks were obtained and the 31 common peaks were displayed in the them by HS-SPME-GC-MS. The 14 samples were classified as 3 grades by cluster analysis and the 10 superior were confirmed to establish the mutual models, as a reference to it, the similarity were calculated between the samples and the mutual models, the results of cluster and similarity analysis remained the same. This research method for evaluating the quality of Magnoliae Flos provides scientific basis. At the same time, this article also for the first time used the highly effective liquid phase chromatography (HPLC) to establish the Magnolia Flos Central Africa volatile constituent HPLC fingerprint, has provided the basis for the quality synthetic evaluation Magnolia Flos raw material for medicine's intrinsic quality. First, in the flowing composition, compared with the separation efficiency which the different flowing composed, determined finally took the flowing by the water and the methyl cyanide. Next in the extraction method, uses the different extraction resolver separately, take the extraction efficiency as goal screening, the definite ethyl acetate to withdraw the resolver finally. Moreover, in detector aspect, compared with evaporation light scattering detector and ultraviolet detector's difference. Compares with the evaporation light scattering detector, because the ultraviolet detector the sensitivity is higher than the peak to be many. Under the optimized chromatograph condition, each batch of Magnolia Flos raw material for medicine's similarity has achieved above 0.9, meets the fingerprint atlas requirement, has provided a fuller scientific basis for its resources quality's appraisal. |
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