The Effects Of Angiostatin From Human Being On Tumor Angiogenesis

Objective:To investigate Angiostatin from human being can inhibit the activity of ascular endothelial growth vascular STT employed protein on mice planting sex lung adenocarcinoma growth transfer inhibition and related mechanism.Methods:Applied affinity chromatography method get plasma fiber by trypsin digestion dissolve enzyme original, chromatography column affinity chromatography, dialysis, get the purified vascular STT employed; Users LEWIS inoculation cell lung cancer in C57BL / 6N coefficient mice, establish mice subcutaneous transplant tumor model. Random tumor-burdened mice will be divided into the treatment group (control group) and many doses, respectively on intraperitoneal injection vascular STT employed and sterile saline. Observe each animal behavior, tumor growth, measuring record tumor-burdened rat mass diameter, surial, Detection each tumor blood essels STT employed with microvascular density (MVD), Using immunohistochemistry flow cytometric analysis line endothelial cell analysis, detecting its biological activities, inoculation mice and compare the tumor size and tumor microvascular density (MVD). Another set the untreated control group and observation vascular STT employed side-effects controls, line lung, liver, kidney pathological examination.Results:Accept vascular STT employed treatment of tumors in mice by mass diameter before treatment (1.368 + 1.17) mg reduced to therapy (148 + 0.46), mg, surial was significantly longer, did not see the obvious side effects. Vascular STT employed mice tumor size less than in control group (P < 0.01), Immunohistochemical detection displayed vascular tumor microvascular employment STT mice density (MVD) lower than that in control group (P < 0. 05).Conclusion:blood vessels STT employed can inhibit angiogenesis and in a certain extent suppress tumor growth. Vascular STT employed for mice, planting sex lung adenocarcinoma have inhibition of growth, this is a vascular tumor tissue employment STT group within angiogenesis is restrained and by a decrease. Vascular employment STT could significantly inhibit C57BL / 6N coefficient LEWIS cell tumor mass in mice lung cancer growth, transfer.