Effects Of Vitmin And Cisplatin On Lewis Lung Cancer Xenograft In Nude Mice

Objective:Resently,lung cancer is one of the biggest threat to human health,and its morbidity and mortality are growing fast.blocking the blood supply of lung cancer research is currently recognized as important research breakthrough in lung cancer.At present,many researches have been done on the mechanism of angiogenesis and the mechanism of vitamin E against angiogenesis.At the same time,it is concluded that the combination of antiangiogenic drugs and chemotherapy drugs can be used to coordinate the anti-tumor effect.we observe the curative effect of vitamin E combined cisplatin in treating Lewis mice tumor ngiogenesis and the effects of vascular endothelial growth factor expression from this experiment,aimed to provide reference for clinical prevention and treatment of lung cancer.Methods:This study used Lewis mouse model of lung cancer.The rats were randomly divided into four groups,each group of 7 mice?The control group(NS group)received daily intraperitoneal injection of saline 90mg/kg;the cisplatin group(DDP group)given daily doses of DDP2mg/kg dissolved in saline as NS group by intraperitoneal injection;vitamin E group(VE group)given daily doses of vitamin E100mg/kg mixed feed in feeding,vitamin E group and cisplatin group(VE+DDP group)received daily vitamin E 100mg/kg+ cisplatin 2mg/kg,Total intraperitoneal cisplatin 3 days and feed vitamin E for 7 days.After twenty-first days of treatment,the mice were sacrificed,we remove tumor tissue then weigh tumor weight,and the tumor weight inhibition rate was calculated.Besides,the tumor sections were stained with HE to observe the tumor angiogenesis,and immunohistochemical staining was used to determine the number of tumor neovascularization and cytokines including vascular endothelial growth factor,angiogenesis and cytokine expression,at the same time,we also extracted the total RNA of tumor tissue,and used to detect expression of reverse transcription-polymerise chain reaction VEGFmRNA and VEGFRm RNA.Results:Groups of tumor weight and tumor inhibition rate of tumor weight comparison:the NS group and VE group tumor growth is the fastest,the tumor growth rate inDDP group compared with NS group and VE group is relatively slow,in NS group and VE group,the tumor weight was significantly greater than the other two groups(P<0.05),which is slightly less than the tumor weight of the VE group in group NS,but the two groups had no significant statistical difference(P>0.05),in DDP group,tumor weight had significant difference with VE+DDP group(P<0.05).Vascular changes in tumor tissue by HE staining: there are a large number of new blood vessels in NS group tumor tissue;microvessel density in tumor tissue was lower in VE group;DDP group showed focal necrosis strip funicular distribution,tissue density is relatively low,the VE+DDP group of tumor cells scattered,visible large areas of necrosis and vascular density is low.The positive expression of vascular endothelial growth factor was detected by immunohistochemistry.The expression of vascular endothelial growth factor in NS group and DDP group was significantly higher than that in the other 2 groups(P<0.05),but there was no significant difference in the expression of vascular endothelial growth factor between the two groups(P>0.05).The expression of VEGF and VEGFR in VE group was significantly lower than that in group DDP(P<0.05),and the expression of VEGF and VEGFR in VE group was not significantly different from that in VE+DDP group(P>0.05).RT-PCR display VEGFmRNA and VEGFRmRNA RT-PCR expression.Statistical analysis showed that the expression of VEGFmRNA and VEGFRmRNA in NS group and PPD group was significantly higher than that in the other two groups(P<0.05),but there was no significant difference between NS and PPD(P>0.05).The expression of VE group was significantly lower than that of group DDP(P<0.05),there was no significant difference between VE group and VE+DDP group(P>0.05).Conclusion: VE has no significant effect on tumor,but can inhibit the expression of VEGF and VEGFR,and the combination of VE and cisplatin can enhance the anti-tumor effect.