| Cinnamomum camphora is an important non-wood and precious timber tree sepcies in south China. The increasingly improved C. camphora industrial train including base construction, crude oil extractive, oil refining, oil purification and oil products development has formed, which will pay more and more important role in increasing foreign exchange and promoting industrial development of flavor. Many researches on C. camphora resourse exploitation were carried out in China, however, the quality of C. camphora oil is not high, and one of the key reasons is the lack of systematic researches about chemical composition of different parts of C. camphora and some bioactive components which are difficult to gasify. Therefore, using different parts of C. camphora as the research object, researches about different types of extractives from a variety of solvents were carried out, and Py-GC/MS and TD-GC/MS technology combined with GC/MS were firstly used to systematically analyze the chemical components in the different solvent extractives of C. camphora different parts, reveal components character and their variation rules, finally identify the bioactive components. This will help to study the prospects of different extractives of different parts of C. camphora, providing scientific base and technological foundation for the high-grade application of C. camphora parts to industries of biomedicine, spicery and commetic, etc.The main results are as follows:(1) The GC/MS analysis of C. camphora extractivesThough MS resolution and function analysis,15 compounds accounting for 98.72% of total peak area from benzene/ethanol extractive of C. camphora wood was identified, the results suggested that the wood extractive contains large amounts of Camphor, Linalyl Propanoate, Eucalyptol, Safrole, etc; 29 compounds accounting for 99.35% of total peak area from benzene/ethanol extractive of C. camphora leaves was identified, the results suggested that the leave extractive contains lots of eucalyptol and alpha-Terpineol, and also contains total 12% of the valuable spice-terpene (alpha.-Pinene, Sabinene, beta.-Pinene, beta.-Myrcene,3-Cyclohexadiene, Caryophyllene, alpha.-Caryophyllene, (+)-Aromadendrene, etc.).(2) The Py-GC/MS analysis of C. camphora extractivesThough Pyrolysis, MS resolution and function analysis,70 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C. camphora twig was identified,110 compounds from 550℃pyrolyzate,76 compounds from 750℃pyrolyzate; 113 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C.camphor a leaves was identified,106 compounds from 550℃pyrolyzate,128 compounds from 750℃pyrolyzate; 136 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C.camphora fruit was identified,201 compounds from 550℃pyrolyzate,161 compounds from 750℃pyrolyzate; 83 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C. camphora old bark was identified,114 compounds from 550℃pyrolyzate, 115 compounds from 750℃pyrolyzate; 64 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C. camphora root bark was identified,53 compounds from 550℃pyrolyzate,53 compounds from 750℃pyrolyzate; 73 compounds from 350℃pyrolyzate of benzene/ethanol extractive of C. camphora tender bark was identified,116 compounds from 550℃pyrolyzate,32 compounds from 750℃pyrolyzate.The variation rule of C. camphora twigs and leaves is similar, under 350℃pyrolysis, the content of ester, hydrocarbon and nitrogenous compounds is more, but the content of ether, terpene, P&S-contained compounds and heterocyclic compounds is less; under 550℃pyrolysis, the content of ester, hydrocarbon and other compounds is more, but the content of ether, carboxylic acid, heterocyclic compounds and P&S-contained compounds is less; under 750℃pyrolysis, the content of hydrocarbon and other compounds is more, but the content of ether, terpene, carboxylic acid, nitrogenous compounds and P&S-contained compounds is less.In different temperature pyrolyzates from benzene/ethanol extractive of C. camphora fruit, under 350℃pyrolysis, the content of hydrocarbon, ester, carboxylic acid, ether and nitrogenous compounds is more, but the content of P&S-contained compounds, heterocyclic compounds, aldehyde and copper, and other conpounds is less; under 550℃pyrolysis, the contents of hydrocarbon, ester, carboxylic acid, and nitrogenous compounds is more, but the contents of ether, terpene, P&S-contained compounds and other compounds is less; under 750℃pyrolysis, the contents of hydrocarbon, carboxylic acid, ester and itrogenous compounds is more, but the contents of ether, terpene, P&S-contained compounds and other compounds is less.In different temperature pyrolyzates from benzene/ethanol extractive of C. camphora old bark, under 350℃pyrolysis, the content of hydrocarbon, ester, carboxylic acid, alcohols and phenol is more, but the content of ether, P&S-contained compounds, heterocyclic compounds and other compounds is less; under 550℃pyrolysis, the contents of hydrocarbon, ester, carboxylic acid, alcohols and phenol is more, but the contents of ether, terpene, P&S-containedcompounds and heterocyclic compounds is less; under 750℃pyrolysis, the contents of hydrocarbon, ester, carboxylic acid, alcohols and phenol is more, but the contents of ether, terpene, P&S-contained compounds and heterocyclic compounds is less.In different temperature pyrolyzates from benzene/ethanol extractive of C. camphora root bark, under 350℃pyrolysis, the content of hydrocarbon, alcohols and phenol, nitrogenous compounds and heterocyclic compounds is more, but the content of ether, ester, carboxylic acid and other compounds is less; under 550℃pyrolysis, the contents of hydrocarbon, carboxylic acid, alcohols and phenol, heterocyclic compounds and other compounds is more, but the contents of ether, terpene and P&S-contained compounds is less; under 750℃pyrolysis, the contents of hydrocarbon, carboxylic acid, heterocyclic compounds, alcohols and phenol is more, but the contents of ether, ester, terpene and other compounds is less.In different temperature pyrolyzates from benzene/ethanol extractive of C.camphora tender bark, under 350℃pyrolysis, the content of hydrocarbon, alcohols and phenol, ester, terpene and other compounds is more, but the content of ether, P&S-contained compounds, aldehyde and ketone is less; under 550℃pyrolysis, the contents of hydrocarbon, alcohols and phenol, terpene, heterocyclic compounds and other compounds is more, but the contents of ether, P&S-contained compounds and carboxylic acid is less; under 750℃pyrolysis, the contents of hydrocarbon, nitrogenous compounds, heterocyclic compounds and other compounds is more, but the contents of ether, ester, terpene, P&S-contained compounds aldehyde and ketone is less.(3) The TD-GC/MS analysis of C. camphora volatilesThough thermal desorption, MS resolution and function analysis,49 compounds from 40℃helium volatiles of C. camphora twig was identified,60 compounds from 60℃Helium volatiles,58 compounds from 80℃Helium volatiles. In different temperature thermal desorption volatiles, the content of hydrocarbon, terpene, heterocyclic compounds and others is more, the content of carboxylic acid, nitrogenous compounds, P&S-contained compounds and ester is very less; during the temperature increasing of 40℃,60℃,80℃, the content of alcohols and phenol, aldehyde and ketone gradually increase, the content of terpene gradually decrease, the variation rule of hydrocarbon and ester is "higher-lower-higher", the variation rule of heterocyclic compounds and other compounds is "lower-higher-lower"The results of determination and functional analysis in this article revealed that the different parts of C. camphora tree can be used to develop high value-added products such as spicery, essence, biomedicine, food, cosmetic, and special industrial materials. |
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