Relationship Of IL-6 And PIAS3 With Liver Regeneration In Cirrhotic Rats After Partial Hepatectomy

Objective:To explore the change of IL-6 and PIAS3 and their correlation with liver regeneration in cirrhotic rats after partial hepatectomy. Methods SD rats were randomized into 2 groups: groupE (cirrhotic rats receiving hepatectomy group ) and group C(normal rats receiving hepatectomy group).we determined liver regeneration rate and expression of proliferating cell nuclear antigen(PCNA), IL-6 mRNA and PIAS3 mRNA in liver tissue at 0,1,2,4,12,24,48and 72 h after operation. Results: liver regeneration rate after partial hepatectomy in group E was significantly lower than in control at 72 h(P<0.05);the expression of PCNA in group E were higher than the group C at 0,1,2,4and 12 h,and got to peak at 48 h, but significantly lower than the group C at 48 h. The levels of IL-6 mRNA in group E incresed slowly, and the peak value was delayed and significantly lower than the group C; The levels of PIAS3 mRNA in group C decreased at 4 hours after operation, the group E decreased at postoperative 12 h , and maintained at a higher level than the group C during the 12 hours. Conclusions: The impaired liver regeneration in cirrhotic liver is associated with low expression of IL-6 and high expression of PIAS3.