Experimental Study On Il-6/stat3 Signaling Pathway In Liver Regeneration Of Rat With Cirrhosis Following Partial Hepatectomy

Objective To evaluate the effect of IL-6/STAT3 signaling pathway in the impaired liver regeneration of cirrhotic rats after hepatectomy.Methods Cirrhosis was induced in rats by administration of CCL4. Untreated rats were used as controls. The control rats as well as the cirrhotic rats were subjected to 70% partial hepatectomy. At 0h/1h/2h/4h/12h/24h/48h and 72h after hepatectomy, the livers and blood were collected , the levels of ALT/AST/ALB/IL-6/TNF-a in serum, the expression of PCNA and active STAT3 proteins in liver remnants were analyzed.Results 24h after hepatectomy , we detected significantly lower mitotic index and PCNA positive cells in the cirrhotic rat as compared to the controls .The cirrhotic rat expressed significantly lower levels of IL-6 as compared to the controls up to 12h post hepatectomy. Significantly lower levels of active STAT3 proteins were also observed in the cirrhotic rat 2h post hepatectomy.Conclusion The impaired liver regeneration in cirrhotic remnants is associated with low activation of STAT3 proteins due to the early low level of IL-6.