Expression Characteristics And Clinical Significance Of RECK In Glioma

Backgrounds and Objectives:Glioma occupies 40-45 percentage of cranial malignancies, which is one of the most difficult-treated tumors in the central nervous system, Normal treatment of glioma is surgical operation accompany with chemo and radiotherapy but the effects still unfavorable with high morbidity, poor prognosis and high mortality for the reason of dangerous location of the tumor, easily local invasion and uncompleted excision of the tumor. Abnormal activation of oncogene and inactivation of anti-oncogene relate to the tumor formation of glioma. The exact mechanism of glioma is still unclear, and the invasive tendency induces high recurrence. Therefore, exploration of genetic alterations closely linked with glioma formation and dissemination would be of great values in prevention, early detection, prognostic evaluation and prolongation live time of this malignancy.Reversion inducing cysteine rich protein with Lazal motifs (RECK) gene, was found in 1998 by Takahashi in fibroblasts of v-Ki-ras transgenic mice. It can inhibit the function of MMP expression and activation. Thus inhibit the invasion and metastasis of malignancies. But there is few corresponding study on glioma.Based on the above problem, the current study aimed to profile expression of RECK during stepwise glioma carcinogenesis and to analyze the relationship to the series of pathological factors through the methods of tissue array and immunohistochemistry staining, the purpose of which is to understand well of the oncological significance.Materials and Methods:Glioma and relative normal brain tissues were obtained from the Anshan City Tumour Hospital. By the methods of paraffin embedded tissue array immuno- histochemistry, the expression pattern of RECK in different glioma tissues were profiled, the correlation between RECK expression and glioma pathology was analyzed. The data were statistically analyzed by Kruskal-Wallis, Mamm Whitney and Spearmen with SPSS 11.5 software.Results:1. 94.7%(18/19)of normal brain tissues express RECK protein however 30.6% (16/59) glioma showed down-regulation of RECK expression, and statistical analyses with Kruskal-Wallis test and Mann-Whitney test revealed the significant differences between the two groups(P=0.046).2. No statistical differences could be observed in different gender, tumor size and pathological types. Significant loss of RECK expression in less than 50 age group compared with that in elder group (P=0.018). I-II stage patients showed higher RECK expression than III-IV stage, significant difference could be established between the two groups (P=0.028). No differences of RECK expression related to patient prognosis and radiotherapy.Conclusion:1. The expression of RECK gene frequently decreases from noncancerous to glioma.2. The frequency of RECK loss of young age and later stage is higher than that of elder group and early stage, but unrelated to factors of gender, tumor size pathological types, survival time and radiotherapy, which indicate relative important ocological significance.