An Preliminary Study On The Effects Of Blueberry And Active Substances On Intestinal Dysbacteria And Intestinal Cancer

Objective:1.Analyze the variation of the cecum index, spleen index and intestinal flora, and discusses the moderating effect of blueberry on mice intestinal flora.2.Analyze the effect of blueberry anthocyanin on the growth of colon cancer cells HT-29 and Caco2, and discusses the inhibition of blueberry anthocyanin on colon-cancer cells.Methods:1.Set up imbalance of intestinal dysbacteria model by treating with ceftriaxone sodium . Using 8g/kg/d doses of ceftriaxone sodium to continuous gavage for 8 days, to establish mice dysbacteria model with monitoring intestinal microflora.2.Observe the change of clinical symptoms, cecum index and spleen index by observing and weighing.3.Analyze the change trend of the 11 species intestinal predominant flora , such as Bifidobacterium,Bacteroides et al, under the normal,intestinal microflora imbalance,natural restoration,blueberry treatment and soybean oligosaccharides treatment.4.Identify anthocyanin by liquid chromatography and test the inhibition effect of the anthocyanin on HT-29 and Caco2.Results:1.To establish stable intestinal dysbacteria model by treating with antibiotics ceftriaxone sodium.2.Compared with the normal group, the cecum index of intestinal dysbacteria group increased significantly(p<0.05); Compared with imbalance group, the cecum index of the natural restoration group did not change remarkably; the cecum index of both blueberry recirculation group and soybean oligosaccharides group decreased remarkably(p<0.05), presenting a recovery trend, and the effects in two groups were similar.3.Compared with the normal group, the spleen index of the flora imbalance groups showed a downtrending but no distinguishing differences; Compared with imbalance group, the spleen index of the natural restoration group did not change remarkably; the spleen index of both blueberry recirculation group and soybean oligosaccharides group increased, presenting a recovery trend, but no distinguishing differences.4.Compared with the normal group, the anaerobic bacteria in dysbacteria group were restrained, and aerobes were bred in great amount. Compared with the dysbacteria group, the types and quantities of the natural restoration group did not change remarkably; the anaerobic bacteria in blueberry treated reappeared, especially the appearances of the Bifidobacteria and Bacteroides predicted that the flora were recovering to normal, the effect of blueberry treatment were similar with that of soybean oligosaccharides.5.Anthocyanin has strong inhibition effect on colon-cancer cell HT-29, and less inhibition on Caco2.Conclusion:1.Blueberry can help the intestinal dysbacteria mice to recover their cecum and spleen.2.Blueberry can regulate the intestinal dysbacteria, its effect is similar with that of soybean oligosaccharides.3.Blueberry anthocyanin has inhibition on colon-cancer cells HT-29 and Caco2.