Expression And Clinical Significance Of CD8~+T Cells In Gastric Carcinoma Tissues

Objective To detect the expression of infiltrating CD8~+T lymphocyte in gastric cancer, gastritis and gastric polyps, compare the differences among them, explore the relationship between the expression of CD8~+T cells in gastric cancer tissue and clinicopathological parameters of gastric cancer.Methods The expression of infiltrating lymphocytes CD8~+T in 102 gastric cancer tissues ,10 gastritis tissues and 10 gastric polyps tissues were detected by using immunohistochemistry assay. Two experienced pathologists determined the results and the quantitative analysis by double blind method. Measurement data was analyzed by using t test and One-way ANOVA test. Survival data was analyzed by using Kaplan-Meier method and Log-rank test.Results CD8~+T cells were expressed both in gastric carcinoma, gastritis and gastric polyp tissues, but the numbers of CD8~+T cells in gastric carcinoma tissues [(29.53±14.71) /HPF] were significantly higher than those in gastritis tissues [(19.54±8.10)/HPF] and polyp tissues [(14.26±6.83) /HPF] (P<0.05). No correlation was found between CD8~+T lymphocyte count and gender, age, tumor location, tumor size, lymph node metastasis, tumor relapse, pathological grade or TNM stage (P>0.05). However, there was a statistically significant correlation between the numbers of CD8~+T cells and the histological type, invasive depth. The CD8~+T cell count was obviously higher in the differentiated group [(33.9±18.4)/HPF] than in the undifferentiated group [(27.6±12.0)/ HPF] (t=2.04, P=0.0439).And the numbers of CD8~+T cells were higher in the non-invasive group [(38.8±13.8)/HPF] than in the invasive group [(27.7±14.1)/HPF] (t=2.45, P=0.0165). The median survival time of gastric cancer patients with CD8~+T cells high expression was 5 months longer than those with low expression, but the difference was not statistically significant. Multi-factor COX model analysis demonstrated that the risk of death in gastric cancer patients was lower in the CD8~+T cells high expression group than in the low expression group (RR=0.82, 95%CI=0.41～1.65).Conclusions Detection of infiltrating lymphocytes CD8~+T expression in gastric carcinoma tissues will be beneficial to the judgment of the prognosis of gastric carcinoma.