Concentration Determination And Significance Of Serum Soluble Co-signalling Molecules SPD-1 And SPD-L1 In Patients With Rheumatoid Arthritis

Objective:Analyse the the relevance between the concentration of serum soluble programmed death 1(sPD-1),soluble programmed death ligands-l(sPD-L1) in patients with rheumatoid arthritis and the evaluation indexes of RA.Explore the latent significance of soluble co-signalling molecules in the process of RA.Methods:60 patients including 30 patients with active RA and 30 with remissive RA,collect whose serum,clinical and examine indicators such as sex,age, disease duration,the patients'visual analogue score(VAS)of pain,morning stiffness,tender joint and swollen joint counts, disease activity score in 28 joints(DAS28),rheumatoid factor (RF),anti-cyclic citrullinated peptide(CCP),erythrocyte sedimentation rate (ESR), C-reactive protein (CRP).health assessment questionnaire scores(HAQ). Choose another 20 healthy people to collect their serum.Measuring the concentration of sPD-1 and sPD-L1 by enzyme linked immunosorbent assay(ELISA), to analyze the relevance between sPD-1,sPD-L1 and evaluation indexes of RA.Results:(1)The concentration of sPD-1 in patients with RA was higher than healthy people statistically (390.41±106.08pg/ml VS 335.00±87.47pg/ml,P<0.05). (2) The concentration of sPD-1 was 417.34±105.35pg/ml in patients with active RA 363.49±101.47pg/ml in patients with remissive RA indicating that the concentration of sPD-1 in patients with active RA was higher than the two other groups(P<0.05). (3)The concentration of sPD-L1 in patients with RA was a little higher than in healthy people, but there was no statistical difference between the two groups (111.88±35.59pg/ml VS 107.43±39.30pg/ml,P>0.05). (4) There was positive correlation between the concentration of sPD-1 and morning stiffness of patients with RA(P<0.05). (5)There was positive correlation between the concentration of serum sPD-1 and sPD-L1 in patients with RA(P<0.05).Conclusions:sPD-1 and sPD-L1 naturally existed in patients with RA and healthy people. We have found that the concentration of sPD-1 was abnormally high in patients with RA, and the concentration of sPD-1 was higher in patients with active RA than in patients with remissive RA, meanwhile there was possitive correlation between sPD-1 and morning stiffness, which indicated that sPD-1 might participate in the pathogenesis of RA.