Expression And Significance Of WWOX And FHIT In The Cervical Cancer

ObjectiveTo discuss the protein expression of the WWOX and FHIT in the squamous cell carcinoma of the cervix , the relationship between these two protein types and the clinicopathological features , lymph node metastasis , prognosis of the squamous cell carcinoma of the cervix( ISCC), and the relevance of these two types of protein.MethodsTo immunohistochemically stain the gene protein expression of WWOX and FHIT in 65 cases of cervical squamous cell carcinoma, 30 cases of adjacent tissues and 30 cases of normal cervical mucosa (a total of 125 cases of paraffin-embedded tissues) with the method of immunohistochemical S-P for the detection of protein expression level in these two indicators. Using the SPSS 12.0 to process the data, Chi-square test and Spearman Correlation analysis to statistically analyze the data.Results1. Expression of WWOX: For the expression of WWOX, there are 0 in of 30 normal cervical mucosa cases show unpositive (0%); 8 of the 30 cases appear to be unpositive in the adjacent tissues (26.7%); 39 are seen to be unpositive in the 65 cases of cervical squamous cell carcinoma (60%). Statistical significance will be found in the differences of these three groups (P<0.01). Protein expression of WWOX are discovered to have relationship with the pathological grades and clinical stage (P<0.01), and will be also influenced by lymph node metastasis (P<0. 05).2. Expression of FHIT: For the expression of FHIT, there are 0 in of 30 normal cervical mucosa cases show unpositive (0%); 7 of the 30 cases appear to be unpositive in the adjacent tissues (23.3%); 43 are seen to be unpositive in the 65 cases of cervical squamous cell carcinoma (66.2%). Statistical significance will be found in the differences of these three groups (P<0.01). Protein expression of FHIT are discovered to have no relationship with the pathological grades, clinical stage and lymph node metastasis (P>0. 05).3. There is no correlation in the expression of WWOX and FHIT in squamous cell carcinoma(P>0.05).Conclusion1. The positive rate of WWOX and FHIT in Squamous Cell Carcinoma of Cervix is significantly lower than that in the adjacent tissues and normal cervical mucosa, which suggests that WWOX and FHIT, genes suppressing tumor, play a role in inhibiting the growth of tumor. By detecting the expression of WWOX and FHIT, a possible new tumor marker may help to diagnose Cervical Cancer.2. The expression of protein WWOX has some relationship with the pathological grade, clinical stage and lymph node metastasis in Squamous Cell Carcinoma of Cervix. Where there is worse degree of differentiation, there is later clinical stage and higher absence of protein WWOX or low expression, suggesting that the absence of gene WWOX may be associated with the occurance development and prognosis of Cervical Cancer.3. The expression of protein FHIT has nothing to do with the pathological grade, clinical stage and lymph node metastasis in Squamous Cell Carcinoma of Cervix, which suggests that the absence of FHIT is only an early event and is not connected with the malignant degree and prognosis of Cervical Cancer.4. There is no correlation in the expression of WWOX and FHIT in squamous cell carcinoma(P>0.05).