| Objective:Isolation and culture of the secretions in skin lesions and its identification of children impetigo from Chengdu. Drug sensitivity test were performed to Staphylococcus aureus. then the sensitive stub and resistance stub to carry on Randomly Amplified Polymorphic DNA fingerprints analysis respectively,carry on the contrast to results.Methods:The bacterias from 178 Impetigo secretions in skin lesions were cultured, the antibiotic sensitivity tests were performed to 21 antibiotics in 162 Staphylococcus aureus and Contrast Analysis Typing of Staphylococcus aureus by Randomly Amplified Polymorphic DNA fingerprints to 162 Staphylococcus aureus.Results:180 strains of bacteria were isolated lesions secretions from 178 children Impetigos of Chengdu, including staphylococcus aureus 162 strains (90.00%), A group of streptococcus pyogenes 4 strains (2.22%), acinetobacter baumannii 4 strains (2.22%), pneumonia crayresearch 4 strains (2.22%), coagulase-negative staphylococcus 2 strains (1.11%), sewers coli 2 strains (1.11%), dung enterococcus 1 strain (0.56%), E.feacalis 1 strain (0.56%),2 cases, among them,lesions was separated to staphylococcus aureus and group Astreptococcus(1.11%). MSSA is 148 strains (91.36%),MRSA is 14 strains (7.64%).Analysis of sensitive in 162Staphylococcus aureus to 21 antibiotics,The sensitivity rates of the Staphylococcus aureus were(from high to low) minocycline(100%), teicoplanin(100%), quinupristin(100%), vancomycin(100%), nitrofurantoin(100%), levofloxacin(98.92%),fusidinsaure(98.92%),rifampicin(97.31%),norfloxacin(94.62%),a mpicil-sulbactam(93.54%),cefotaxime(92.60%),cefazolinum(92.60%),cefuroxim(91.98 %),gentamicin(90.12%),oxacilin(89.51%),tetracycline(85.19%),chloramphenicol(82.10 %),cotrimoxazol(15.43%),clindamycin(15.43%).erythromycin(6.17%),penicillin(0.62%) .Analysisofresistantin 162Staphylococcusaureusto21antibiotics,TheresistantratesoftheSta phylococcusaureuswere(fromhightolow),penicillin(99.38%),erythromycin(93.83%),clin damycin(84.57%),cotrimoxazol(84.57%),tetracycline(14.81%),chloramphenicol(11.73% ),oxacilin(10.50%),gentamicin(9.26%),cefazolin(8.40%),cefotaxime(8.40%),cefuroxim(8 .02%),ampicil-sulbactam(5.55%),nitrofurantoin(4.32%),levofloxacin(1.23%),rifampicin( 0.62%),minocycline(0%),teicoplanin(0%),quinupristin(0%),vancomycin(0%),nitrofurant oin(0%),fusidinsaure(0%).Analysis of sensitive in 148 Staphylococcus aureus to 21 antibiotics,The sensitivity rates of the Staphylococcus aureus were(from high to low) minocycline(100%), teicoplanin(100%),quinupristin(100%),vancomycin(100%),nitrofurantoin(100%),cefotax ime(100%),cefazolinum(100%),ampicil-sulbactam(99.32%),cefuroxim(99.32%),,levoflo xacin(98.65%),fusidinsaure(98.65%),rifampicin(97.97%),norfloxacin(93.24%),gentamin (89.86%),oxacilin(89.51%),chloramphenicol(86.48%),tetracycline(85.81%),clindamycin (16.22%), cotrimoxazol(15.54%), erythromycin(6.76%),penicillin(0.68%).148 strains Meticillinsensitive staphylococcus aureus in 21 antibiotics resistance from high to low in turn for penicillin (99.32%), erythromycin (93.24%), cotrimoxazol(84.46%), clindamycin (83.78%), tetracycline fosomycin (14.19%), gentamycin (9.46%), chloromycetin (8.11%), norfloxacin(4.73%), oxacilin (2.03%), levofloxacin (1.35%), cephalosporins (0.68%), rifampicin (0.68%). fusidinsaure, ampicillin sulbactam, cefazolin, cefotaxime, nitrofurantoin, vancomycin, quinupristin, teicoplanin, minocycline are not shown the resistance.14 strains Meticillin resistant staphylococcus aureus to 21 antibiotics sensitivity rate from high to low in turn is minocycline (100%), teicoplanin (100%), quinupristin (100%), vancomycin(100%), nitrofurantoin(100%), levofloxacin (100%), fusidinsaure (100%), rifampicin (100%), norfloxacin(100%), gentamycin (92.86%), tetracycline (78.57%) and ampicillin sulbactam (35.71%), chloromycetin (35.71%), cefotaxime (14.29%),cefazolin(14.29%), cefuroxim(14.29%),cotrimoxazol(14.29%), clindamycin (7.15%). Among them, oxacilin, erythromycin, penicillin all showed that the resistance.14 strains Meticillin resistant staphylococcus aureus to 21 antibiotics resistance from high to low in turn for penicillin (100%), erythromycin (100%), oxacilin(100%), clindamycin(92.86%),cefotaxime(85.71%),cefazolinum(85.71%), cefuroxim(85.71%), cotrimoxazol(85.71%) and ampicillin sulbactam (64.29%)), chloromycetin (57.14%), tetracycline (21.43%) and gentamycin (7.14%). Among them, minocycline, teicoplanin, quinupristin, vancomycin, nitrofurantoin, levofloxacin, fusidinsaure, rifampicin and norfloxacin were not show drug resistance. 148 strains Meticillin sensitive staphylococcus aureus and 14 strains Meticillin resistant staphylococcus aureus to 21 antibiotics by statistical analysis, the difference Resistance Rate was statistically significant (t=2.866, P< 0.05), pustular lesions from male and female children of chengdu isolated staphylococcus aureus in 21 antibiotic resistance by statistical analysis, the difference was not statistically significant (t=0.639, P> 0.05).162 strains of staphylococcus aureus Randomly Amplified Polymorphic DNA finger printing, obtain DNA 5-11 bands, the band 250bp-2000bp molecular size, is divided into 8 genotypes, namely, genotypeⅠfor 3 strains (1.85%), genotypeⅡfor 43 strains (26.54%), genotypeⅢfor 51 strains (31.48%), genotypeⅣfor 7 strains (4.32%), genotypeⅤfor 10 strains (6.17%), genotypeⅥfor 42 strains (25.93%), genotypeⅦfor 3 strains (1.85%), genotypeⅧfor 3 strains (1.85%). Among them, to genotypesⅢ, genotypesⅡand genotype VI mostly, the three genotype 65.43%(106/162).148 strains Meticillin sensitive staphylococcus aureus genotype respectively is: genotypeⅠfor 3 strains (2.03%), genotypeⅡfor 33 strains (22.30%), genotypeⅢfor 50 strains (33.78%), genotypeⅣfor 7 strains (4.73%), genotypeⅤfor 10 strains (6.76%), genotype VI for 39 strains (26.35%), genotypeⅦfor 3 strains (2.03%), genotypeⅧfor 3 strains (2.03%). Among them, to genotypeⅢ, genotypes VI, genotypeⅡmostly, three genotypes of total 82.43%(122/148).14 strains Meticillin resistant staphylococcus aureus genotype respectively is: genotypeⅡ10 strains, genotype VI 3 strains, genotypeⅢ1 strain. Among them, genotypeⅡgive priority, on the genetic type of total 71.43%(10/14), followed by genotypesⅥ, total 21.43%(3/14).Conclusions:Pathogenic bacteria from Children impetigo secretions in skin lesions of Chengdu is most staphylococcus aureus (90.00%), to give priority to minocycline, teicoplanin, quinupristin, vancomycin and nitrofurantoin highly sensitive (sensitive rate all for 100%). And for penicillin, erythromycin, clindamycin and cotrimoxazol are height resistance (84.57% above). MSSA to cefotaxime, cefazolin, cefuroxim, ampicillin sulbactam highly sensitive (sensitive rate was 100%,100%,99.32%,99.32% respectively), while MRSA, for cefotaxime, cefazolin, cefuroxim, ampicillin sulbactam highly resistant (resistance rate was 85.71%.85.71%,85.71%,64.29%respectively).The antibiotic resistant is higher MRSA than MSSA to 21 kinds of antibiotics from Children impetigo of Chengdu. difference was statistically significant.162 strains staphylococcus aureus Randomly Amplified Polymorphic DNA finger printing, is divided into 8 genetic types, the genetic typeⅢmost, followed by genotypesⅡ, genotypesVI, these three genotypes are 65.43 %of the total(106/162).148 strains MSSA genotype have eight types, with genotypeⅢ(50strains,33.78%), gene genotypeⅥ(39 strains,26.35%), genotypeⅡ(33 strains,22.30%) mostly, these three genotypes of total 82.43%(122/148),14 strains MRSA only genotype 3 genotypes, respectively genotypeⅡ10 strains (71.43%), genotype VI 3 strains (21.43%), genotypeⅢ1 strain (7.14%), genotypeⅡprimarily. |
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