And -141. Microrna-21 Expression In Epithelial Ovarian Cancer Tissue And Serum And Clinical Significance Discussed

Objective:To investigate the expression of microRNA-21 and microRNA-(miR-21 and miR-141) in epithelial ovarian cancer samples and to investigate the possibility of serum miR-21 and miR-141 used as biomarker for epithelial ovarian cancer.Methods:The expression of miR-21 in tissues and serum from 20 cases of epithelial ovarian cancer and the expression of miR-141 in tissues and serum from 16 cases of epithelial ovarian cancer and 4 cases of normal persons and in serum from 4 cases of benign epithelial ovarian tumor was detected by real-time RT-PCR.Results:The real-time RT-PCR method was sensitive and specific. The relative expression level of miR-21 in the serum of patients with epithelial ovarian cancer was significantly higher than those in benign epithelial ovarian tumor and normal persons (P<0.05). The relative expression level has no significant relationship between benign epithelial ovarian tumor and the normal persons (P> 0.05).The expression of miR-21 was not correlated to the progression of the clinical stages, histological grading and lymphatic metastasis (P> 0.05). The expression of miR-21 in patients'serum and tissues of CA125≤200U/ml was significantly higher these of CA125> 200U/ml(p<0.05).The expression of miR-141 in epithelial ovarian cancer tissues was significantly higher than those in normal persons (P<0.05). The expression of miR-141 in epithelial ovarian cancer serum was significantly higher than those in benign epithelial ovarian tumor and normal persons (P<0.05), but there was no difference between the latter two groups. The expression of miR-141 increased with the progression of the clinical stages (p<0.01), but it had not correlation with histological grading and the increasing of CA125(P> 0.05). There was no difference for miR-141 expression with clinicpathologic features in tissues of patient of epithelial ovarian cancer (P>0.05).Conclusion:MiR-21 and miR-141 might play a role as an oncogene in the occurrence and development of ovarian epithelial carcinoma. miR-21 and miR-141 have higher sensibility and have potential as a new marker of early diagnosis for epithelial ovarian cancer.