Effects Of 6-O-carboxymethyl-chitosan On The Expression Of TGF-β₁ And CTGF In Human Hypertrophic Scar Fibroblasts

Objective:To observe the effects of 6-O-carboxymethyl-chitosan (6-O-CMC) with different concentrations on the expression of transforming growth factor-beta1 (TGF-β1) and connective tissue growth factor(CTGF) in human hypertrophic scar fibroblasts (HSFBs) in vitro.Methods:HSFBs were cultured in vitro, and the 3-5 generation cells were chose to the next experiments. The DMEM medium with different concentrations of 6-O-CMC (100,200,400ug/ml) were used in the experimental groups, and the control group without 6-O-CMC. After 24 hours, cell morphology and growth state of HSFBs were observed under the microscope. After 48 hours, the expression of TGF-β1 and CTGF protein was detected by ELISA. The expression of TGF-β1 mRNA and CTGF mRNA was was detected by Real-time RT-PCR.Results:Compared with the control group, the expressions of HSFBs TGF-β1 and CTGF protein in the other groups all reduced obviously (p<0.05). and the expressions of HSFBs TGF-β1 and CTGF mRNA in the other groups also reduced distinctly(p<0.05). Concentration and expression were in inverse proportion(p<0.05). The expression of TGF-β1 or CTGF protein and mRNA are on the same direction.Conclusions:6-O-CMC could suppress the expression of HSFBs TGF-β1 or CTGF. We exploring the mechanism of 6-O-CMC in treating hypertrophic scar, which provides theoretical basis for the development and utilization of new drugs.