Experimental Study Of The NV-KS Abstracted From Panax Notoginseng Which Inhibit The Lung Adenocarcinoma Cell Line XWLC-05from Yunnan Xuanwei

[Objective]:This study was designed to primarily investigate the inhibitory effects and the mechanisms of NV-KS abstracted from Panax Notoginseng of Yunnan on lung cancer cell line XWLC-05and to eluminate its potential mechanism in apoptosis.[Methods]:1. MTT (Methy thiazolyl tetrazolium) assay was used to evaluate the inhibitory effects of XWLC-05,GLC and KMB-17induced by NV-KS and CDDP in different time and different concentration, and each one is divided into four groups of negative control group,solvent control group, and experimental group(with the NV-KS), positive control group(with the CDDP),and then to calculate the half inhibiting concentration(ICso).2. XWLC-05were induced by NV-KS and CDDP in72hours,KeyGEN living cells/apoptotic cells/necrotic cells identification kit(AO/EB method)by fluorescence microscopy was used to observate apoptosis of the cells.3. XWLC-05were induced by NV-KS and CDDP in72hours and then put in fixed liquid, electron microscope was used to scan the cells.4. DNA ladder of XWLC-05and KMB-17cells were induced by NV-KS and CDDP in72hours was tested by GENMED DNA testing kit.5. All data were analyzed by SPSS17.0software and statistics were expressed as mean±standard deviation (χ±s), single factor analysis of variance (ONE of WAY ANOVA) and t test were used to get the statistical significance.[Results]:1. NV-KS and CDDP could inhibit the proliferation of XWLC-05, GLC and KMB-17cells, on dose-and time-dependenant manner in vitro, the values of IC50for each group are exhibited in following table:There is no statistic significancance between cisplatin and pairwise group (p>0.05), but abvious difference of IC50was found in NV-KS cells betwen XWLC-05and KMB-17treatment (p=0.012; p<0.05).2. The apoptosis XWLC-05cells induced by NV-KS and CDDP at72hours were found by fluorescence microscope with AO/EB method.3. Apoptotic bodies of XWLC-05induced by NV-KS and CDDP at72hours were deteced by electron microscopy images.4.. DNA ladders were found in XWLC-05and KMB-17cells treated with GENMED.[Conclusion]:1. NV-KS could inhibit the proliferation of the XWLC-05cells in vitro, its proliferation is on time and dose dependment.2. NV-KS could inhibit the proliferation of the XWLC-05cells in vitro,apoptosis in the early formation of crescent-shaped cells, and also the formation of apoptosis in late cell cytoplasm bud-like protuberances.3. NV-KS could inhibit the proliferation of the XWLC-05cells in vitro, apoptotic bodies of cells can form.4. NV-KS can induce XWLC-05cells apoptosis and form fracture bands, but to KMB-17it can not form the fracture bands within a certain concentration.