Expressions And Significances Of Chemokine Receptor CXCR4and Its Ligand CXCL12in Bladder Tumor Tissue

To explore the expressions of chemokine receptor CXCR4and its ligand CXCL12in bladder carcinoma and normal bladder tissue. To analyze the relationship between the expressions of CXCR4and its ligand CXCL12with the clinical pathological features in bladder tumor. To evaluate their roles and clinical significances in carcinogenesis, invasion and metastasis of carcinoma, to provide a new molecular target for the intervention therapy of bladder carcinoma.Methods:The expressions of CXCR4and CXCL12at cell-level were detected by S-P immunohistochemical method in42cases of bladder carcinoma and normal bladder tissue. The mRNA expressions of CXCR4and CXCL12mRNA were detected at transcription level by reverse transcription-polymerase chain reaction(RT-PCR). The protein expressions of CXCR4and CXCL12proteins were detected by Western blot.Results:Immunohistochemistry showed that the positive expressions of CXCR4and CXCL12in bladder carcinoma tissue were significantly higher than that in the normal bladder tissue (z=7.332,6.758,P<0.001). Their expressions were closely correlated with the histological differentiation and depth of invasion of the cancer was involved (z=2.598-4.032, P<0.05), but not associated with gender and age of the patients (2=0.273-0.554, P>0.05). There was a positive correlation between the expressions of CXCR4and CXCL12in the cancer (r=0.661, P<0.05). From the point of view of the level of mR NA, CXCL12/CXCR4did not express in normal bladder tissue, the expressions in advanced bladder cancer were higher, there were statistically significant differences(F=56.642,67.928, P<0.01). From the point of view of the level of protein, CXCL12/CXCR4did not express in normal bladder tissue, the expressions in advanced bladder cancer were higher, there were statistically significant differences t(F=59.453,61.732, P<0.01).Conclusion:Chemokine receptor CXCR4and its ligand CXCL12play a role in the development of bladder tumor. The high expressions of CXCR4and CXCL12promote the invasion of the bladder tumor cells. CXCR4and CXCL12have some correlations between the expressions, suggesting that high expressions of CXCR4in cancer cells,accelerate the evolution of bladder tumor by the chemotactic effect of...