Expression And Significance Of Chemokine Receptor CXCR4in Human Bladder Carcinoma Cell Lines

Objective:To compare the differences of proliferation,migration and invasion between three bladder cacinoma cell lines,to explore the effects of proliferation,migration and invasion by SDF-1in these cells,detecting the expressions of CXCR4in different bladder cacinoma cell lines, to investigate the relationship between the expressions of CXCR4and the abilities of proliferation migration and invasion in these cells, to find out new experimental data and target in the treatment of bladder cacinoma.Methods:By MTT method, detecting the proliferative abilities between different bladder cacinoma cell lines, to observe the influences of cell proliferative ability after using SDF-1. By scratch test, detecting the migratory abilities between three cell lines,to observe the influences of cell migratory ability after using SDF-1.By cellular invasion assays,to detect the invasion abilities between three cell lines, relationship was analyzed between invasion and the action of SDF-l.By semi-quantiative reverse transcription polymerase chain reation,the expressions of CXCR4mRNA in three bladder cacinoma cell lines were determined at the level of mRNA.By Western Blot,the expressions of CXCR4protein in three bladder cacinoma cell lines were determined at the level of protein.Results:Using MTT method,the absorption value of EJ-M3experimental group(0.424±0.016) was greater than the control group(0.345±0.015)(p<0.01),the absorption value of EJ experimental group(0.393±0.018) was greater than the control group(0.332±0.023)(p<0.05),the absorption value of BIU-87experimental group(0.357±0.014) was greater than the control group(0.312±0.017)(p<0.05),there were significantly differences among the groups(p<0.05).By cell scratch experiment, the number of cell migration to the scratch area at12h and24h of EJ-M3experimental group(32.18±2.65,43.18±3.88) was greater than the control group(9.00±1.78,19.34±3.40)(p<0.01),the number of EJ experimental group(30.68±2.51,41.82±2.78) was greater than the control group(8.51±1.51,18.68±1.52)(p<0.05),the number of BIU-87experimental group(23.67±2.81,34.51±2.75) was greater than the control group(8.18±1.48,17.01±1.42)(p<0.05),there were significantly differences among three cell lines(p<0.05); By Transwell experiment, the penetrating cell number of EJ-M3experimental group(83±6) was greater than the control group(55±6)(p<0.01), the number of EJ experimental group(52±5) was greater than the control group(33±4)(p<0.05),the number of BIU-87experimental group(29±5) was greater than the control group(11±3)(p<0.05),there were significantly differences among three cell lines(p<0.05); By semi-quantitative reverse transcription polymerase chain reaction,the mRNA of CXCR4was expressed in three cell lines, the relative optical density was EJ-M3(1.3357±0.1435)>EJ (0.7662±0.1338)>BIU-87(0.1658±0.0677),the differences were significant between three cell lines(p<0.05).By Western Blot, the protein of CXCR4expressed in three cell lines, the relative optical density was EJ-M3(0.5257±0.1338)>EJ(0.3246±0.1038)>BIU-87(0.0858±0.0631), the differences were significant between three cell lines(p<0.05).Conclusion:The biological characteristics of three bladder cacinoma cells were different, it was found that the abilities of proliferation migration and invasion in three bladder cacinoma cells were different, the abilities of proliferation, migration and invasion in bladder carcinoma cells were promoted by using SDF-1.The expression of CXCR4is positively correlated with cells' proliferation,migration and invasion capability,CXCR4/CXCL12(SDF-1) axis plays an important role in the invasion and metasitasis of bladder cacinoma,it will become a new target to treat bladder cacinoma.