Study Of Chemosensitivity Of Tumar Cell From Primary Tumors And Liver Metastases With Orthotopic Implant Models Of Human Gastric Carcinoma In Vitro In Nude Mice

Chemotherapy is important in comprehensive treatments of gastriccarcinoma, especially in the patients with primary tumors resected, which isthe most important therapeutic means. However, the chemotherapy isapproving on and on, it is unable to choose chemotherapy individualized yet,partly because of the different sensitivity among drugs and patients.Undoubtedly this is one of the most important reasons to lead to chemotherapyfail, therefore individualized chemotherapy becomes the hot spot ofchemotherapy study.Objective: Using orthotopic implant and metastatic models of humanstomach cancer in nude mice and sulforhodamine B assay, to study thechemosensitivity differences of tumor cells between primary gastric carcinomaand hepatic metastasis to seven kinds of chemotherapy drugs(5-Fu, CDDP,MTX, LOHP, eADM, MMC, VCR).Methods: Experiments were performed on30nude mice. Tumor cellline SGC-7901of human gastric carcinoma was inoculated subcutaneouslyinto nude mice. After transplanted tumor had developed, its cells wereinoculated subcutaneously to next normal nude mice and passed repeatedlyuntil solid tumor was formed. Then SGC-7901tumor block from the sixthgeneration was pasted into mouse stomach wall in greater curvature withcopious blood vessels by OB organism glue. When these animals showedphysical signs of exhaustion and was dying, they were sacrificed. Take theprimary tumor of stomach and liver metastases. Then in vitro SRB assay wasperformed on these primary tumors (PT) and liver metastases (LM).Statistical analysis was performed using SPSS13.0software package.Data was dealt with t test and Spearman correlation analysis. P<0.05was considered significant for all statistical analyses.Result: There was significantly different chemosensitivity to antitumordrugs between PT and LNMs in4/7drugs (VCR,L-OHP, eADM, MMC, allP<0.05). The inhibition rates of eADM and MMC were higher in LM thanthose in PT (all P<0.05), whereas the inhibition rates of tumor cells in LM forL-OHP and VCR were lower than those in PT.(all P<0.05). No significantlydifference of inhibition ratio between PT and LM was found in5-Fu,CDDPand MTX(all P>0.05). There was a significantly positive correlation betweenthe inhibition rates of PT and LM for5-Fu, L-OHP, MTX (r=0.3851~0.5203,all P<0.05). While there was no significantly correlation between theinhibition rates of PT and LM for CDDP,eADM,MMC and VCR.Conclusion:1. Intact tissue block of human stomach cancer can beimplanted into gastric wall of nude mouse by using organism glue. Thismetastatic model can well present clinical metastatic process of human gastriccarcinoma and be useful for the study of its growth, metastasis and treatment.2. All7drugs have low inhibition rates for gastric carcinoma cells, and itshows that it's unrealistic to control this cancer with one drug.3.Heterogeneity of chemosensitivity to most antitumor drugs is shown in livermetastases in gastric cancers (eADM,MMC,L-OHP,VCR).