The Level And Clinical Significance Of Leptin And Adiponectin In Childhood Acute Leukemia

Objective：In the past20years, the incidence of obesity increased rapidly.Incidence and mortality rates of some diseases those were closely related toobesity, such as diabetes, cardiovascular disease, hypertension, metabolicsyndrome and cancer were rising. Scientists paid more and more attention tothe research of the adipose tissue which played a very important role of theobesity, and they had made a breakthrough. In the research, they found that theadipose tissue was not only a tissue of energy storage, but also had a lot ofimportant endocrine function, and produced a variety of complex materials.These substances were known as the fat cells factor. They could work in localtissue or various systems of the body. Leptin and adiponectin research of fatcell factor was one of the earliest and most thorough. The present researchfocused on the relationship among leptin, adiponectin and various kinds ofnoumenal tumors, such as breast cancer, liver cancer, colon cancer, prostatecancer and other solid tumors. But there was a short of the research in therelationship between fat cell factor and the leukemia, and there was even lessto confer of the combined action mechanism and clinical significance betweenfat cell factor and leukermia. The research for patients with leukemia in vivoleptin level changes were relatively little.The purpose of this topic wasapplicated double antibody sandwich enzyme-linked immunosorbent assaytechnology (ELISA) to detect the level of serum leptin and adiponectin indifferent periods of chemotherapy in childhood acute leukemia, and to exploreits clinical significance.Methods:1Cases and groups：This research was randomly selected30cases newlydiagnosed and untreated with childood acute leukemia to be hospitalized in theblood group of department of padiatrics in the second hospital of Hebei medical university from Aug2010to Aug2011, those treated as the untreatedgroup of the experiment. Among them there were16children with acutelymphoblastic leukemia (ALL), and the other14children with acute myeloidleukemia (AML). After a month from first chemotherapy, all the30cases hadachieved complete remission,which was treated as Remission1month Group.Then after3months with treatment observation, they reached continuedcomplete remission, and treated them as Remission3months Group.Meanwhile, as the1:1matching principle, choosed the healthy children withsame factor like gender, age, body height and weight as control group. All thechildren in the research should have not any disease of heart, brain, kidney andendocrine. They also had not the sexual development.2Sample collection, handling and the data measurement: All of objects wereextracted2ml fasting venous blood in the morning of7:00to8:00. Allvenous blood were placed in the promoting coagulation tubes, and then putthem in the ordinary centrifugal machine, with a speed of2500r/min after10min, serum samples were collected. Divided the samples equally and keptthem in the fridge at-80℃. Measured the level of leptin and adiponectin ofserum. Measured the standing height and the weight of the children. Tookdown the result with the unit of kg and cm, the accuracy rating are0.1kg and0.1cm. The body mass index (BMI)=Body weight(kg)/the square of thebody height(m2).3Statistics analysis: SPSS13.0was used, and the data was described asmean±SD. Analysis methods include Chi-square test, two-sample/group t-test,analysis of variance. P value of less than0.05was considered as statisticalsignificant.Results:1Influencing factor: The differences of age, gender and BMI among fourgroups had not statistics significance (P>0.05).2Serum leptin levels: Leptin level was (1.08+0.62) ng/ml in untreatedgroup, the level was(3.64+0.76) ng/ml in remission1month group, the levelwas (4.91±0.89) ng/ml in remission3month group. The level of leptin in untreated group was the lowest. The level of leptin increased gradually withprolonged treatment time. The differences among three groups had statisticalsignificance (P<0.05); The level was (5.07+0.87)ng/ml in normal controlgroup, and the level had not statistical significance between remission3monthgroup and normal control group(P>0.05).3Serum adiponectin levels: Adiponectin level was group(8.85±2.05)ng/ml inuntreated group, the level wa(s13.17±2.36)ng/ml in Remission1month group,the level was(16.05±2.93)ng/ml in remission3months group. The level ofadiponectin in untreated group was the lowest. The level of adiponectinincreased gradually with prolonged treatment time. The differences amongthree groups had statistical significance(P<0.05); The level was(16.18±3.44)ng/ml in normal control group,and the level had not statistical significancebetween remission3month group and normal control group(P>0.05).Conclusion:1The levels of serum leptin and adiponectin in untreated group was distinctlylower than the control group, so they could become a kind of detection indexof acute leukemia.2After complete remission in childhood acute leukemia, the levels of serumleptin and adiponectin gradually rose to normal. We suggested that dynamicdetection the levels of serum leptin and adiponectin in childhood acuteleukemia in different stages of treatment were contributived to judgedevelopment of disease and assess treatment effect.